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  • Development And Validation Of UV HPLC Method For The Estimation Of Diacerein In Bulk And Tablet Dosage Form

  • 1Department of Pharmaceutical quality Assurance, R. G, Sapkal College of Pharmacy, Anjaneri, Nashik-422212
    2Department of Pharmaceutical Chemistry, R. G, Sapkal College of Pharmacy, Anjaneri, Nashik-422212
    3Department of Pharmacology, R. G, Sapkal College of Pharmacy, Anjaneri, Nashik-422212

Abstract

Objective: Objective of the present analytical research work was to develop and validate Spectrophotometric method and High Performance Liquid Chromatographic method (HPLC Method) for the Diacerein bulk and tablet dosage form Methods: A spectrophotometric method and a HPLC method have been developed and validated for estimation of Diacerein in bulk. Method A (UV SPECTROMETRY Method): Methanol was used for the preparation of stock and working standard solutions of the drugs. 400-200nm UV range was used to scanned standard solutions of drugs using UV spectrophotometer. The ?max of Diacerein was found to be 248 nm. Method B (HPLC Method): The HPLC Method for Diacerein was developed using Cosmosil C18 (4.6mm x 250mm, Particle size: 5µm), as stationary particle, isocratic mode. Methanol : Water (80:20v/v) pH3as mobile phase. Mobile phase was maintained at a flow rate of 1 ml/min and detection was carried out at 248 nm. Both the methods were validated in accordance with ICH guidelines Results: Diacerein was found to be linear in the concentration range of 5-25 µg/ml for spectrophotometric and HPLC method. Retention time was found to be 4.3 min for Diacerein. Interpretation and Conclusion: Results of validation study were found to be satisfactory. So, the methods can be successfully applied for the routine analysis of Diacerein.

Keywords

UV Spectrophotometric Method, HPLC Method, Diacerein

Reference

  1. ICH, Q2 (R1); Validation of Analytical Procedure: text and methodology. International Conference on Harmonization, Geneva, 2005; 1-13.
  2. Beckett AH and Stenlake JB. Practical Pharmaceutical Chemistry, Part-II, 4th Ed., New Dehli; CBS Publishers and Distributors, 2004; 85- 174.
  3. Willard HH, Merrist LL, Dean AJ, Frank A and Settle AF. Instrumental Methods of Analysis, 7th Ed., New Dehli; CBS Publishers and Distributors, 1986; 2-3, 585-587
  4. Skoog DA, Holler FJ and Nieman TA. Principle of Instrumental Analysis, 5th Ed., Thomson Publication Limited, London, 2004; 103.
  5. Sethi PD., High Performance Liquid Chromatography, Quantitative Analysis of Pharmaceutical Formulations, 1st edition, New Delhi; CBS Publishers and Distributors, 2001; 3-11, 116-120.
  6. Toomula N, Kumar A, Kumar S, Bheemidi VS. Development and Validation of Analytical Methods for Pharmaceuticals & Bioanalytical Techniques, 2011; 2(5): 1-4.
  7. Lakshmi SRP. A Review on Chromatography with High Performance Liquid Chromatography (HPLC) and its Functions. Research and Reviews: Journal of Pharmaceutical Analysis, March 2015; 4(1): 1-15.
  8. Swartz ME. HPLC: An introduction and review. J Liq Chromatogr Rel Technol. 2005;25:1253–63.
  9. Giannellini V, Salvatore F, Bartolucci G, Coran S A, Bambagiotti-Alberti M. (2005) A validated HPLC stability-indicating method for the determination of diacerhein in bulk drug substance. J Pharm Biomed Anal 39: 776.
  10. Keddal G. Lalitha,   Thangavel Venkatachalam, A Simple HPLC Method for Quantitation of Diacerein in Tablet Dosage Form, Eurasian J Anal Chem 2010;5(1):81–88.
  11. Deshpande M. M, Estimation of Diacerein by UV Spectrophotometry in Bulk and formulation,International Journal of Chemical and Analytical Science 2011,2(1),1-2
  12. N. Kannappan, Madhukar A, Analytical Method Development And Validation Of Diacerein Tablets By Rp-Hplc, Ijcrgg, Jan-Mar 2010, Vol.2, No.1, Pp 143-148.
  13. Narendra Nyola, Govinda Samy Jeyabalan, Development and Validation of a RP-HPLC Method for Simultaneous Estimation of Diacerein and Aceclofenac in Pharmaceutical Dosage Form, RRJPA,Vol 1, Issue 1, Oct–Dec, 2012
  14. https://go.drugbank.com/drugs/DB11994
  15. https://pubchem.ncbi.nlm.nih.gov/compound/Diacerein

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Amol Amrutkar
Corresponding author

Department of Pharmaceutical quality Assurance, R. G, Sapkal College of Pharmacy, Anjaneri, Nashik-422212

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Smita Aher
Co-author

Department of Pharmaceutical Chemistry, R. G, Sapkal College of Pharmacy, Anjaneri, Nashik-422212

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Rishikesh Bachhav
Co-author

Department of Pharmacology, R. G, Sapkal College of Pharmacy, Anjaneri, Nashik-422212

Amol Amrutkar, Smita Aher, Rishikesh Bachhav, Development And Validation Of Uv-Hplc Method For The Estimation Of Diacerein In Bulk And Tablet Dosage Form, Int. J. in Pharm. Sci., 2023, Vol 1, Issue 2, 94-103. https://doi.org/10.5281/zenodo.6220056

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