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Abstract

This study's major goal is to create an antifungal herbal shampoo with curry leaves as the key ingredient, which are known to strengthen hair follicles and encourage hair growth. This herbal shampoo attempts to improve hair growth, strength, and natural darkening while preventing hair loss, a frequent hair condition1. An efficient and secure shampoo formulation was created by combining curry leaves with additional natural ingredients including amla, aloe vera gel, neem, Reetha, Shikakai1. The shampoo is made to clean hair by eliminating dandruff, sebum, and filth without the dangers of chemical compounds that are frequently included in commercial shampoos. There are many different kinds of shampoo, both liquid and solid, but this article focuses on a natural herbal liquid shampoo2,3.

Keywords

Shikakai, Reetha, Amla, Neem, Tulsi, Aloe vera gel..

Introduction

Growing worries about the negative effects of synthetic substances frequently present in personal care products have led to an increase in the demand for herbal cosmetic formulations worldwide in recent years2. There has been a notable movement in the hair care industry toward herbal substitutes that offer long-term advantages, safety, and effectiveness. In order to improve scalp health and hair vitality, two shampoos and hair treatment formulas are increasingly including botanical components, which are recognized for their therapeutic qualities3. Hair is the fundamental element of human attractiveness. Herbs have been used for hair management, cleaning, and decoration since ancient times6. As time has passed. Despite the substantial market share of synthetic agents, people are becoming aware of their negative effects impacts on hair, eyes, and skin2. Herbal shampoo is a kind of cosmetic preparation made with natural plant materials called herbs. Any hair washing product made with extracts from herbs can be referred to as "herbal shampoo." Another way to think of it is as a thick liquid hair care solution that cleanses and gets rid of oil and dirt from hair5.

    1. Poly-herbal antifungal shampoo

A hair-cleaning product made with extracts from two or more medicinal plants with antifungal qualities is called a polyherbal antifungal shampoo. It is intended to cure or prevent fungal illnesses including seborrheic dermatitis and dandruff while cleaning the hair and scalp. Herbal components including neem, reetha, shikakai, amla, and tulsi, which offer organic cleaning, antibacterial, and conditioning properties, are frequently found in these shampoos. The development of fungi that cause scalp infections is inhibited by the combined action of many herbs. Polyherbal antifungal shampoos are popular for preserving scalp health and enhancing hair quality since they are often thought to be safer and gentler than synthetic shampoos.

1.2 Function of poly shampoo

  • Removes dirt, excess oil, dust, sweat, and pollutants from hair and scalp.
  • Reduce fungal infection (like dandruff, Malassezia)
  • Prevent bacterial scalp infection.
  • Prevents scalp dryness and maintains natural moisture.
  • Cleans scalp pores and promotes healthy hair growth.
  • Improves hair texture.
    1. Difference of poly-herbal shampoo and synthetic shampoo

Polyherbal Shampoo

  1. Made from medicinal plant such as neem, amla, reetha, and shikakai.
  2. Contains natural ingredients and herbal active compounds.
  3. Usually milder and safer for hair and scalp.
  4. Provides additional benefits like antifungal, anti-dandruff, and hair nourishment.
  5. Produces less foam because it uses natural surfactants.

Synthetic Shampoo

  1. Prepared using chemical ingredients such as SLS, parabens, and silicones.
  2. Contains artificial surfactants, preservatives, and fragrances.
  3. Mainly used for strong cleansing and high foaming action.
  4. May cause side effects like scalp irritation, dryness, hair fall, or allergies.
  5. Produces more foam due to synthetic detergents.

1.4Anatomy and Physiology of Hair

The fatty layer of the scalp contains follicular units from which hair develops. Each unit has one to four hairs and a hair bulb at the base that generates hair cells that are fed by dermal blood vessels. As hair develops and separates from the skin, its outer layer solidifies into keratin6.

There are three primary stages of hair growth:
(1) Anagen (Growth Phase): Hair production is active and can continue for years.
(2) Catagen (Transitional Phase): A brief stage during which hair growth ceases.
(3) Telogen (Resting Phase): New growth starts and hair loses.

2.Classification Of Shampoo7

A. Based on the appearance:

  • Powder shampoo
  • Liquid shampoo
  • Gel shampoo
  • Oil shampoo
  • Dry shampoo

B. Based on their origin:

  • Herbal shampoo
  • Synthetic shampoo

C. Based on their function:

  • Conditioning shampoo
  • Anti dandruff shampoo
  • Baby shampoo
  • Clarifying shampoo

3.Advantages of poly-herbal shampoo

  • Cleansing properties, improving hair hygiene.
  • Gentle on scalp and suitable for long-term use.
  • Herbal ingredients reduce the chances of itching, irritation, redness or hair damage.
  • Herbal ingredients are biodegradable.
  • Less environmental toxic load compared to fully synthetic shampoo.
  • Many herbal ingredients are easily available and inexpensive.
  • People nowadays prefer natural and herbal products, so polyherbal shampoo have high market demand.

4.MATERIALS AND METHODS

Materials -

 

Table4.1- Ingredients

S.N.

Ingredients

Quantity (50ml)

Properties

1

Reetha

5g

Natural cleanser / Surfactant

2

Shikakai

5g

Hair cleanser / Conditioner

3

Amla

5g

Antioxidant

4

Neem

5g

Antifungal / Antibacterial

5

Tulsi

2.5g

Antimicrobial

6

Aloe vera gel

2.5ml

Moisturizer and Conditioner

7

Sodium lauryl sulphate

5g

Foaming Agent

8

Citric Acid

0.1g

Ph Adjuster

9

Methyl Paraben

0.05g

Preservative

10

Distilled Water

Up to 50ml

Solvent

 

 Methodology

 

  1. Take 5g each of reetha, shikakai, amla, neem, tulsi powder.

 

 
  1. Add about 50ml of distilled water, soak overnight.

 

  1. Boil for 15-20 minutes, cool, and filter.

 

 

 

 

 

 
  1. Collect filtrate. (this is combined herbal extract)

 

 

 

 

 

 
  1. Take 25 ml distilled water in a clean beaker.

 

 
  1. Add sodium lauryl sulphate (SLS) slowly with gentle stirring to form a uniform solution (avoid foam).

 

 

 

 

 
  1. Add aloe vera gel, mix well.

 

 

 

 

 
  1. Add reetha, shikakai, amla, neem, tulsi extracts one by one to the base with gentle stirring.

 

 

 
  1. Continue stirring until a uniform blend is obtained.

 

 

 
  1. Dissolve methyl paraben in a small amount of warm water, then add to the mixture.

 

 

 
  1. Adjust pH between 5.5-6.5 using citric acid solution.

 

 
  1. Prepared shampoo

 

 

 

                                                                       

  1. Transfer the prepared shampoo into a clean, dry, labeled bottle.

 

 

 
  1. Store in a cool, dark place.
 

5. RESULT

The formulated polyherbal shampoo was evaluated to ensure its effectiveness, stability, and consumer acceptability. The tests performed included physical appearance, pH determination, foaming, and viscosity determination. These evaluations are necessary to verify the effectiveness and safety of the shampoo by comparing it with marketed formulations.

  1. Physical appearance

The prepared formulations were assessed for fluidity, clarity, and capacity to produce foam.

Table 1. physical appearance

Color

Brownish color

Odor

Characteristic

Transparency

Not transparent

Texture

Smooth

  1. pH determination

2 mL of the polyherbal shampoo solution was taken into a Petri dish. After that, a pH litmus paper was dipped into the 2 mL solution. The observed pH was found to be 5.5 to 6.5.

 

 

 

Fig. pH determination

  1. Foaming test

To evaluate the foaming ability, the cylinder shake method was used. 1.5 mL of polyherbal shampoo was taken in a 10 mL graduated cylinder. The cylinder was covered by hand and shaken 10–12 times. After shaking, the foam produced was measured.

 

 

Fig. Foaming Test

  1. Viscosity determination

Viscosity influences the spreading ability of shampoo and its ease of application. The viscosity of the prepared shampoo was determined at 25°C using an Ostwald viscometer.

  1. Microbial test

A small amount of the prepared shampoo was taken and stored at different temperatures. However, no microbial growth was observed in the prepared shampoo.

Table 2. Evaluation parameter

Evaluation parameter

Inference

Color

Brownish color

Odor

Characteristic

Transparency

Not transparent

Texture

Smooth

pH determination

5 – 6

Microbial activity

No microbial growth

Table 3. Stability test

Parameter

Room temperature

Color

No change

Odor

No change

DISCUSSION

The polyherbal shampoo showed satisfactory performance with acceptable appearance, consistency, and cleansing ability. The natural ingredients contributed to effective hair cleaning while being gentle on the scalp. Adequate foaming and stability were observed, indicating a well-balanced formulation. Overall, it offers a safe and eco-friendly alternative to synthetic shampoos.

CONCLUSION

Poly-herbal shampoo formulation and assessment is a complicated procedure that necessitates careful consideration of several elements. These include choosing the herbal powders or components needed for the formulation and assessing the efficacy and safety of the finished product. There are a number of possible advantages to using herbal components in shampoo composition, including better hair health and less of an influence on the environment. To guarantee the product's efficacy and safety, however, appropriate testing and assessment are crucial. All things considered, the development and assessment of herbal shampoo is a crucial field of study that may provide customers a more sustainable and natural hair care choice.

The current study's goal was to create a herbal shampoo that guards against fungal infections. Furthermore, compared to chemical conditioning treatments, it is safer. The shampoo's pH was modified to preserve the scalp's acidic environment. Shampoo is made with a variety of plant powders or herbal substances, including neem, reetha, shikakai, amla, and aloe vera.

REFERANCES

  1. Kale, K., & Somani, S. (2023). Formulation and evaluation of herbal shampoo for curry leaves. J. Herb. Res., 12(3), 45–52.
  2. Jadhav, S. R., et al. (2024). A review on Murraya koenigii for hair growth promotion. Int. J. Ayurvedic Herb. Med., 15(1), 67–78.
  3. Sharma, P., & Mehta, V. (2023). Antioxidant properties of amla and its potential in hair care formulations. Int. J. Cosmet. Sci., 11(2), 88–99.
  4. Satyanarayana, T., Nirmala, D., Sai, C., Dileep, K., Sai, K., Akshitha, M., Sucharitha, V., & Sagarika, M. (2022). Formulation and evaluation of polyherbal shampoo powder. International Journal of Pharmacy and Pharmaceutical Research. Available at: www.ijppr.humanjournals.com
  5. More, A. G., Pote, P. D., Kore, P. S., & Garhwani, Y. D. (2022). Formulation and evaluation of polyherbal anti-dandruff shampoo. International Journal of Ayurvedic Medicine, 13(2), 365–369. Available at: http://ijam.co.in https://doi.org/10.47552/ijam.v13i2.2256
  6. Singh, S., & Maury, S. (2015). Formulation and evaluation of polyherbal antidandruff powder shampoo using pomegranate seed and guava leaves. World Journal of Pharmaceutical Research, 11. https://doi.org/10.20959/wjpr20226-24088
  7. Stenn KS, Paus R. Controls of hair follicle cycling. Physiol Rev 2001;81(1):449–494. https://doi.org/10.1152/physrev.2001.81.1.449
  8. Lodha G. Formulation and evaluation of polyherbal shampoo to promote hair growth and provide antidandruff action. J Drug Deliv Ther 2019;9(4-A):296–300. https://doi.org/10.22270/jddt.v9i4-A.3529
  9. Mainkar AR, Jolly CI. Formulation of natural shampoos. Int J Cosmet Sci 2001;23:59–62. https://doi.org/10.1046/j.1467-2494.2001.00055.x
  10. Ishii MK. Objective and instrumental methods for evaluation of hair care product efficacy and substantiation of claims. In: Hair and hair care. New York: Marcel Dekker, Inc; 1997. p. 261–302. https://doi.org/10.1201/9780203719565-10
  11. Khaloud Al Badi, Shah A. Khan. Formulation, evaluation and comparison of the herbal shampoo with the commercial shampoos. Beni-Suef Univ J Basic Appl Sci 2014;3:301–305. https://doi.org/10.1016/j.bjbas.2014.11.001
  12. Latha M, Ramkumar M, Pari L, et al. Phytochemical and antimicrobial study of Scoparia dulcis L. J Med Food 2006;9(3):391–394. https://doi.org/10.1089/jmf.2006.9.391
  13. Manikar AR, Jolly CI. Evaluation of commercial herbal shampoos. Int J Cosmet Sci 2000;22(5):385–91. https://doi.org/10.1046/j.1467-2494.2000.00050.x
  14. Khaloud Al Badi, Shah A. Khan. Formulation, evaluation and comparison of the herbal shampoo with the commercial shampoos. Beni-Suef Univ J Basic Appl Sci 2014;3(4):301–305. https://doi.org/10.1016/j.bjbas.2014.11.001
  15. Zambare KK, et al. Preparation and evaluation of polyherbal shampoo. Res J Top Cosmet Sci 2019;10(2):41–44. https://doi.org/10.5958/2321-5844.2019.00008.5
  16. Utami SM, Djajadisastra J, Saputri FC. Using hair growth activity, physical stability, and safety tests to study hair tonics containing ethanol extract of licorice (Glycyrrhiza glabra Linn.). Int J Appl Pharm 2017;9:44–48. https://doi.org/10.22159/ijap.2017v9i4.19311
  17. Aghel N, Moghimipour B, Dana RA. Formulation of a herbal shampoo using total saponins of Acanthophyllum squarrosum. Iran J Pharm Res 2007;6(3):167–172. https://doi.org/10.22037/ijpr.2010.717 (brieflands.com)
  18. Hay RJ, Graham-Brown RA. Dandruff and seborrhoeic dermatitis: Causes and management. Clin Exp Dermatol 1997;22(1):2–6. https://doi.org/10.1046/j.1365-2230.1997.d01-171.x
  19. Tarun J, Susan J, Susan VJ, Criton S. Evaluation of pH of bathing soaps and shampoos for skin and hair care. Indian J Dermatol 2014;59(5):442–444. https://doi.org/10.4103/0019-5154.139861
  20. Tegeli VS, et al. Formulation and evaluation of polyherbal shampoo containing different herbal extract. Res J Top Cosmet Sci 2022;13(2):87–91. https://doi.org/10.5958/2321-5844.2022.00015.2
  21. Manikar AR, Jolly CI. Evaluation of commercial herbal shampoos. Int J Cosmet Sci 2000;22(5):385–91. https://doi.org/10.1046/j.1467-2494.2000.00050.x
  22. Harrison JL, Davis KD. Cold-evoked pain varies with skin type and cooling rate: A psychophysical study in humans. Pain 1999;83:123–135. https://doi.org/10.1016/S0304-3959(99)00175-0
  23. Randall VA. Is alopecia areata an autoimmune disease? Lancet 2001;358:1922–1924. https://doi.org/10.1016/S0140-6736(01)06855-6
  24. Maffei C, Fossati A, Rinaldi F, et al. Personality disorders and psychopathologic symptoms in patients with androgenetic alopecia. Arch Dermatol 1994;130:868–872. https://doi.org/10.1001/archderm.1994.01690080080025
  25. Wolfram LJ. Human hair: A unique physicochemical composite. J Am Acad Dermatol 2003;48:S106–S114. https://doi.org/10.1067/mjd.2003.139
  26. Khaloud Al Badi, Shah A. Khan. Formulation, evaluation and comparison of the herbal shampoo with the commercial shampoos. Beni-Suef Univ J Basic Appl Sci 2014;3:301–305. https://doi.org/10.1016/j.bjbas.2014.11.001
  27. Ranganathan S, Mukhopadhyay T. Dandruff: The most commercially exploited skin disease. Indian J Dermatol 2010;55:130–134. https://doi.org/10.4103/0019-5154.74535
  28. Hay RJ, Graham-Brown RA. Dandruff and seborrhoeic dermatitis: Causes and management. Clin Exp Dermatol 1997;22:2–6. https://doi.org/10.1046/j.1365-2230.1997.d01-171.x
  29. Aghel N, Moghimipour E, Raies Dana A. Formulation of a herbal shampoo using total saponins of Acanthophyllum squarrosum. Iran J Pharm Res 2010;6(3):167–172. https://doi.org/10.22037/ijpr.2010.717 (brieflands.com)
  30. Barrera-Rojas CH, Otoni WC, Nogueira FTS. Shaping the root system: The interplay between miRNA regulatory hubs and phytohormones. J Exp Bot 2021;72(20):6822–6835. https://doi.org/10.1093/jxb/erab299

Reference

  1. Kale, K., & Somani, S. (2023). Formulation and evaluation of herbal shampoo for curry leaves. J. Herb. Res., 12(3), 45–52.
  2. Jadhav, S. R., et al. (2024). A review on Murraya koenigii for hair growth promotion. Int. J. Ayurvedic Herb. Med., 15(1), 67–78.
  3. Sharma, P., & Mehta, V. (2023). Antioxidant properties of amla and its potential in hair care formulations. Int. J. Cosmet. Sci., 11(2), 88–99.
  4. Satyanarayana, T., Nirmala, D., Sai, C., Dileep, K., Sai, K., Akshitha, M., Sucharitha, V., & Sagarika, M. (2022). Formulation and evaluation of polyherbal shampoo powder. International Journal of Pharmacy and Pharmaceutical Research. Available at: www.ijppr.humanjournals.com
  5. More, A. G., Pote, P. D., Kore, P. S., & Garhwani, Y. D. (2022). Formulation and evaluation of polyherbal anti-dandruff shampoo. International Journal of Ayurvedic Medicine, 13(2), 365–369. Available at: http://ijam.co.in https://doi.org/10.47552/ijam.v13i2.2256
  6. Singh, S., & Maury, S. (2015). Formulation and evaluation of polyherbal antidandruff powder shampoo using pomegranate seed and guava leaves. World Journal of Pharmaceutical Research, 11. https://doi.org/10.20959/wjpr20226-24088
  7. Stenn KS, Paus R. Controls of hair follicle cycling. Physiol Rev 2001;81(1):449–494. https://doi.org/10.1152/physrev.2001.81.1.449
  8. Lodha G. Formulation and evaluation of polyherbal shampoo to promote hair growth and provide antidandruff action. J Drug Deliv Ther 2019;9(4-A):296–300. https://doi.org/10.22270/jddt.v9i4-A.3529
  9. Mainkar AR, Jolly CI. Formulation of natural shampoos. Int J Cosmet Sci 2001;23:59–62. https://doi.org/10.1046/j.1467-2494.2001.00055.x
  10. Ishii MK. Objective and instrumental methods for evaluation of hair care product efficacy and substantiation of claims. In: Hair and hair care. New York: Marcel Dekker, Inc; 1997. p. 261–302. https://doi.org/10.1201/9780203719565-10
  11. Khaloud Al Badi, Shah A. Khan. Formulation, evaluation and comparison of the herbal shampoo with the commercial shampoos. Beni-Suef Univ J Basic Appl Sci 2014;3:301–305. https://doi.org/10.1016/j.bjbas.2014.11.001
  12. Latha M, Ramkumar M, Pari L, et al. Phytochemical and antimicrobial study of Scoparia dulcis L. J Med Food 2006;9(3):391–394. https://doi.org/10.1089/jmf.2006.9.391
  13. Manikar AR, Jolly CI. Evaluation of commercial herbal shampoos. Int J Cosmet Sci 2000;22(5):385–91. https://doi.org/10.1046/j.1467-2494.2000.00050.x
  14. Khaloud Al Badi, Shah A. Khan. Formulation, evaluation and comparison of the herbal shampoo with the commercial shampoos. Beni-Suef Univ J Basic Appl Sci 2014;3(4):301–305. https://doi.org/10.1016/j.bjbas.2014.11.001
  15. Zambare KK, et al. Preparation and evaluation of polyherbal shampoo. Res J Top Cosmet Sci 2019;10(2):41–44. https://doi.org/10.5958/2321-5844.2019.00008.5
  16. Utami SM, Djajadisastra J, Saputri FC. Using hair growth activity, physical stability, and safety tests to study hair tonics containing ethanol extract of licorice (Glycyrrhiza glabra Linn.). Int J Appl Pharm 2017;9:44–48. https://doi.org/10.22159/ijap.2017v9i4.19311
  17. Aghel N, Moghimipour B, Dana RA. Formulation of a herbal shampoo using total saponins of Acanthophyllum squarrosum. Iran J Pharm Res 2007;6(3):167–172. https://doi.org/10.22037/ijpr.2010.717 (brieflands.com)
  18. Hay RJ, Graham-Brown RA. Dandruff and seborrhoeic dermatitis: Causes and management. Clin Exp Dermatol 1997;22(1):2–6. https://doi.org/10.1046/j.1365-2230.1997.d01-171.x
  19. Tarun J, Susan J, Susan VJ, Criton S. Evaluation of pH of bathing soaps and shampoos for skin and hair care. Indian J Dermatol 2014;59(5):442–444. https://doi.org/10.4103/0019-5154.139861
  20. Tegeli VS, et al. Formulation and evaluation of polyherbal shampoo containing different herbal extract. Res J Top Cosmet Sci 2022;13(2):87–91. https://doi.org/10.5958/2321-5844.2022.00015.2
  21. Manikar AR, Jolly CI. Evaluation of commercial herbal shampoos. Int J Cosmet Sci 2000;22(5):385–91. https://doi.org/10.1046/j.1467-2494.2000.00050.x
  22. Harrison JL, Davis KD. Cold-evoked pain varies with skin type and cooling rate: A psychophysical study in humans. Pain 1999;83:123–135. https://doi.org/10.1016/S0304-3959(99)00175-0
  23. Randall VA. Is alopecia areata an autoimmune disease? Lancet 2001;358:1922–1924. https://doi.org/10.1016/S0140-6736(01)06855-6
  24. Maffei C, Fossati A, Rinaldi F, et al. Personality disorders and psychopathologic symptoms in patients with androgenetic alopecia. Arch Dermatol 1994;130:868–872. https://doi.org/10.1001/archderm.1994.01690080080025
  25. Wolfram LJ. Human hair: A unique physicochemical composite. J Am Acad Dermatol 2003;48:S106–S114. https://doi.org/10.1067/mjd.2003.139
  26. Khaloud Al Badi, Shah A. Khan. Formulation, evaluation and comparison of the herbal shampoo with the commercial shampoos. Beni-Suef Univ J Basic Appl Sci 2014;3:301–305. https://doi.org/10.1016/j.bjbas.2014.11.001
  27. Ranganathan S, Mukhopadhyay T. Dandruff: The most commercially exploited skin disease. Indian J Dermatol 2010;55:130–134. https://doi.org/10.4103/0019-5154.74535
  28. Hay RJ, Graham-Brown RA. Dandruff and seborrhoeic dermatitis: Causes and management. Clin Exp Dermatol 1997;22:2–6. https://doi.org/10.1046/j.1365-2230.1997.d01-171.x
  29. Aghel N, Moghimipour E, Raies Dana A. Formulation of a herbal shampoo using total saponins of Acanthophyllum squarrosum. Iran J Pharm Res 2010;6(3):167–172. https://doi.org/10.22037/ijpr.2010.717 (brieflands.com)
  30. Barrera-Rojas CH, Otoni WC, Nogueira FTS. Shaping the root system: The interplay between miRNA regulatory hubs and phytohormones. J Exp Bot 2021;72(20):6822–6835. https://doi.org/10.1093/jxb/erab299

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Praveen Kumar Sahu
Corresponding author

Rungta Institute of Pharmaceutical Science & Research, Bhilai

Photo
Kamlesh Patel
Co-author

Rungta Institute of Pharmaceutical Sciences, Bhilai

Photo
Suchita Wamankar
Co-author

Rungta Institute of Pharmaceutical Science & Research, Bhilai.

Photo
Dr Gyanesh Kumar Sahu
Co-author

Rungta Institute of Pharmaceutical Science & Research, Bhilai.

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Dr Chanchal Deep Kaur
Co-author

Rungta Institute of Pharmaceutical Sciences, Bhilai

Kamlesh Patel, Praveen Kumar Sahu, Suchita Wamankar, Dr Gyanesh Kumar Sahu, Dr Chanchal Deep Kaur, Formulation and Evaluation of Polyherbal Antifungal Shampoo, Int. J. of Pharm. Sci., 2026, Vol 4, Issue 3, 3830-3837, https://doi.org/10.5281/zenodo.19332664

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