1Department of Pharmaceutical Quality Assurance, K.B.H.S.S. Trust Institute of Pharmacy, Malegaon Camp, Nashik.
2Principal and Professor in Pharmacology, K.B.H.S.S. Trust Institute of Pharmacy, Malegaon Camp, Nashik.
A collection of techniques called chromatography are employed to extract constituents from a mixture. This approach is divided into two phases; stationery and moveable. The basis for the constituent separation is the variation in the partition coefficients between the two phases. The Greek words "graphein" (to write) and "chroma" (color) are the origin of the word "chromatography". High performance liquid chromatography (HPLC) is an essential technology for determining the quality and quantity of pharmaceutical and environmental samples. It is the most flexible, safe, trustworthy, and efficient chromatographic method available for assessing the caliber of pharmaceutical components. Each component in a mixture can be identified, quantified, and separated using the analytical chemistry technique of high-performance liquid chromatography (HPLC; formerly known as high-pressure liquid chromatography). A liquid is used as the mobile phase in HPLC, one type of liquid chromatography. Reversed-phase HPLC is the type most frequently employed. The mobile phase is relatively polar in a reversed-phase system, while the stationary phase is comparatively non-polar. The instruments used in HPLC analysis include a solvent reservoir, pump, injector, column, detector, integrator, or acquisition and display system, and detector. The system's brain is the column where separation occurs. Among other things, HPLC is useful for identifying, measuring, and resolving compounds.
Chetna Malwal, P. B. Jadhav, Vinod Bairagi, A Comprehensive Review on High-Performance Liquid Chromatography (HPLC), Int. J. of Pharm. Sci., 2024, Vol 2, Issue 1, 288-297. https://doi.org/10.5281/zenodo.10477121
10.5281/zenodo.10477121
