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  • Phytochemical And Pharmacological Activity Of Tridax Procumbems Linn
  • Department Of Pharmacognosy RJS College Of Pharmacy, Kopargaon, Ahemdnagar, Maharastra, India

Abstract

India has been blessed with an abundance of medicinal herbs primarily because of the country's climate and seasons, which promote the growth of various plant species. Tridax procumbens is a typical medicinal herb that is generally found in India as a weed and pest plant. It is classified as part of the Asteraceae family and is commonly referred to as Coat Button, Kansari (Hindi), or Ghamara (local language). The most valuable medicament utilized for the manufacturing of compounds described in Ayurvedic literature is Tridax procumbens. It is mostly used as an anticoagulant, an antifungal, and a wound healing agent insect repellent, dysentery and diarrhea in Indian traditional remedies. And their extract has various pharmacological properties (anti-inflammatory, hepatoprotective, immunomodulating, antimicrobial or antibacterial, antiseptic, anticancer, repellent, hemostatic, ant diabetic, urolithiasis, blood pressure lowering, antioxidant, bradycardia, etc.). The chemical components of the plant showed that its leaves contain various alkaloids, flavonoids, carotenoids, fumaric acid, etc. The synthesis of aromatic substances in the plant results in its primary secondary metabolites, such as the phenol derivative and oxygen oxidation. It is also active against antimicrobial agents such as gram-positive and gram-negative bacteria. It is also used as an adsorbent for chromium. Here we try to focus on the broad phytochemical and pharmacological activities of Tridax Procumbens.

Keywords

Tridax Procumbens, Herb, Phytochemical, Pharmacological Plant Extracts, Medicinal plant.

Introduction

Tridax procumbens could be a species of blossoming plant within the daisy (Asteraceae) family. It is best known as a broad weed and bug plant. The plant is local of tropical America and naturalized in tropical Africa, Asia, and Australia. It could be a wild herb dispersed all through India. It is yearly or biennial to some degree patently hispid herbs. Stem branched, inching at base, sub erect or trailing over.[1]  Tridax procumbens is best known as a far reaching weed and bother plant. T. procumbens is additionally known  as 'Mexican daisy' (in Mexico), 'Coat button' and 'Tridax daisy' (in  English), 'Jayanti Veda' (in Sanskrit), 'Gharma' (in Hindi), 'Dagadipala'  (in Marathi), 'Vettukkaaya-thalai' (Tamil/Siddha) and 'Akala kohadi'  (in society).[14] It is frequently establishing at hubs with singular, long stalked, yellow composite, heterogamous, promiscuous blooms with white blooming heads and exceptionally shaggy, coarsely toothed, petislate, praise or lanceolate takes off.[13] It is found in street sides, squander ground, railroads, riverbanks, glades. This plants wide spread conveyance and significance as a weed due to the spreading steam and inexhaustible seed generation.[2,3]  Tridax procumbens is found to have pharmacological exercises like hepatoprotective impact, immunomodulating property, promising wound recuperating movement, antidiabetic, hypertensive impact, antimicrobial, creepy crawly repellent action, against incendiary and antioxidant, bronchial catarrh, diarrhea, loose bowels moreover anticipate falling of hairs and leads to hair development advancement.[1] Flavonoids, fundamental oils, saponins, tannins, steroids, alkaloids, carbohydrates, carotenoids, terpenoids, and other chemical substances have been separated and recognized from this species.[14]

Scientific classification of Tridax procumbens: [3,16]


Table 1: Classification of Tridax procumbens


       
            A.png
       

    


The complete plant and seed being utilized to treat an assortment of afflictions the clears out are cooked and eaten as a vegetable. But no Pharmacognostical work has been done so distant. Subsequently, an endeavor has been made to consider the Pharmacognostic parameters on the takes off of Tridax procumbens L.[13]


Table 2 :  Plant information of Tridax Procumbens Linn.


       
            Screenshot 2024-05-29 181946.png
       

    



       
            Picture1.jpg
       

    

Fig 1 A. Whole Plant


       
            Picture2.png
       

  

Fig  1 B. Tridax Flower

       
            Picture3.jpg
       

    Fig 1 C Tridax Leaves


MATERIAL AND METHODS:

Collection of Medicinal Plant (Tridax Procumbens):

The takes off of Tridax procumbens were collected from Western Ghats, Tamilnadu, India. T.Procumbens was transported to the research facility for distinguishing proof and a example was put away for assist thinks about. It was shade dried for 10 days and powdered employing a blender processor. The leaf powder was utilized for the extraction of phytochemicals. [4]

APPARATUS AND CHEMICALS: [1]

Soxhlet extractor, forceps clamp stand, heating mantle, three round bottomed jar, reflux condenser, heating mantle and chiller, TLC plate, column for chromatography, refining (Distilled) device iodine chamber and test tubes. Ethanol, Hexane, Ethyl acetic acid derivation (ethyl acetate), Silica gel, Pet ether and distilled water.

Methods for Extractions:

1. By Macerations: [9]

  • For both phytochemical and pharmacological screening, the powder must be extricated utilizing water.
  • The maceration prepare is vital for fluid extraction.
  • We were given 20gm of Tridax procumbens powdered takes off in a measuring utensil.
  • After completely mixing, include 5ml of chloroform to anticipate any organism from developing. Utilize a muslin cloth to appropriately seal the measuring utensil after that.
  • For seven days, the measuring utensil was altogether mixed each 2-3 hours. On the seventh day, the arrangement was sifted. By applying warm from exterior, the filtrate will be dissipated.
  • The filtrate was vanished till the strong molecule was gotten.
  • In arrange to do a phytochemical and pharmacological screening, the strong molecule ought to be collected and adequately weakened.

2. By Soxhlet Extractions: [9]

  • For ethanolic extraction by utilizing the Soxhlet device, we taken around 10gm of powdered takes off of Tridax procumbens into the Soxhlet device.
  • Included the powder test in a permeable pack or “thimble” made of solid channel paper or a muslin cloth, which is put in chamber of the Soxhlet device.
  • Chloroform will evacuate greasy acids and chlorophyll display within the powder test.At that point included 250ml of Chloroform in device and begin the water supply.
  • Begun the warming mental and put at 600C and proceeded supply the warm till the drop of dissolvable from the siphon tube does not take off buildup when dissipated.
  • Stopped the warming supply and hold up for cool the gathering.
  • Collected the powder from permeable pack or thimble and dry it appropriately at room temperature. After drying powder once more included it in permeable pack or thimble.
  • Included the 250ml of Ethanol within the get together and begin the water supply. Begun the warming mental and put at 600C.
  • Proceeded supply the warm till the drop of dissolvable from the siphon tube does not take off buildup when dissipated.
  • Halt the warming supply and hold up for cool the get together. Taken dissolvable collected at circular foot carafe and go for vanishing.
  • Dissipated the dissolvable till we accomplish the strong particulate. Scratch and weaken it and go for phytochemical and pharmacological screening.

C. Soxhlet gadget and extraction done with 100 ml of particular solvents (moo (low) polar to tall (High) polar) like as hexane, butanol, chloroform, ethanol and water. [7]

PHYTOCHEMICAL SCREENING (COVERING) OF TRIDAX PROCUMBENS: [3,9,10]


Table 3: Qualitative test for T.procumbens Linn.


       
            Screenshot 2024-05-29 182014.png
       

    


PRELIMINARY PHYTOCHEMICAL SCREENING (COVERING) OF T.PROCUMBENS:

The distinctive organic dissolvable extracts of Tridax procumbens were utilized to screen the taking after phytochemicals like sugar, alkaloids, phenolic compounds, Flavonoids, tannins, saponins, amino acids and ascorbic acids. Chemical tests were carried out on the watery extricate (Extract) on the powdered examples utilizing standard methods. [7]

Thin Layered Chromatography:

[1] Thin layer chromatography is done as employing a thin, uniform layer of silica gel coated onto a piece of glass. The stationary stage for thin layer chromatography too frequently contains the mobile stage could be a appropriate fluid dissolvable or mixture of solvents. The RF value for each compound is at that point worked out utilizing the equation. (Mobile Phase: 7ml Hexane: 3ml Acetone) [8].

Equation for Thin layer chromatography:

RF Value (Retention Factor): Distance travelled by solute / Distance travelled by solvent.

PHARMACOLOGICAL ACTIVITY OF TRIDAX PROCUMBENS LINN:

The chemical constituents of the plant appeared that its leaves contain different alkaloids, flavonoids, carotenoids, fumaric corrosive (acid) etc. Their extricate has different pharmacological properties (anti-inflammatory, hepatoprotective, immunomodulatory, antimicrobial or antibacterial, movement sterile, anti-cancerous movement, repellency movement, hemostatic movement, antidiabetic, anti-urolithiatic action, hypotensive, antioxidant effect, bradycardiac effects, Wound Healing etc.) [1]



       
            Screenshot 2024-05-29 182153.png
       

   


CONCLUSION:

Tridax procumbens contains a number of valuable constituents such as flavone glycoside, chromone glycoside, bithiophenes, flavonoid (procumbenetin), sterols, terpenoids, lipids and polysaccharides, phenolic content, tannins, catechins, and various secondary metabollites with significant pharmacological activities such as anti-inflammatory, antibacterial, wound-healing,  and antioxidant activity, anti cancer, antistaphylococcal, apoptotic effect. Apart from this, it also possesses antimicrobial and immunomodulatory action, which provides the basis for further studies.

REFERENCES:

  1. Anil saini & dr.parvesh gupta. Phytochemical studies of the leaves of Tridax procumbens. International journal of research and analytical reviews. [Volume 5 i issue 3 i July– sept 2018]. E issn 2348 –1269, print issn 2349-5138.
  2. Minal Wani, Snehal Pande and Nitin More. Callus induction studies in Tridax procumbens L. International Journal of Biotechnology Applications, ISSN: 0975–2943, Volume 2, Issue 1, 2010, pp-11-14.
  3. Vikram mahadev shingade and nisha nitish karandikar. A review on pharmacological &biological activity of tridax procumbens linn (bhringraj). World journal of pharmaceutical research. Volume 10, issue 6, 705-715. Issn 2277– 7105.
  4. Asad syed  , natarajan benit  , abdullah  alyousef  , abdulaziz alqasim , mohammed arshad. In-vitro antibacterial, antioxidant potentials and cytotoxic activity of the leaves of tridax procumbens. A. Syed et al. / saudi journal of biological sciences 27 (2020) 757–761.
  5. Awasthi , irshad m, das m.k., ganti s.s , moshahid  a. Rizvi. Anti-inflammatory activity of calotropis gigantea and tridax procumbens on carrageenin-induced paw edema in rats. Ethnobotanical leaflets 13:568-77, 2009.
  6. A. Shanmugapriya and s. Maneemegalai. Phytochemical screening, antimicrobial and antioxidant activity of leaf extracts of tridax procumbens. International journal of research in pharmacy and chemistry (ijrpc) 2017, 7(3), 320-326. Issn: 2231?2781.
  7. Valivittan, k and p. Dhasarathan. Screening of phytochemicals and antimicrobial activity in tridox procumbens. Rising research journal publication.global j. Of mod. Biol. & tech., 2014: 4 (1). Issn 2277-6656 (online): 2231-5179.
  8. Durga devi.m, banu.n. Study of antioxidant activity of chlorophyll from some medicinal plants. Indian journal of research. Volume: 4 | issue : 2 | feb 2015. Issn - 2250-1991.
  9. Ashutosh bangale, dr. Sangeeta tanavade, akash bhosale, abhishek bagadi, priyanka bandichhode. Phytochemical analysis & antiinflammatoryactivity of tridax procumbens l. International journal of creative research thoughts (ijcrt)2308025. © 2023 ijcrt | volume 11, issue 8 august 2023 | issn: 2320-2882.
  10. Ammar maali , methaq n algabr , najeeb yl al-surmi , aziza m taj aldeen , hussein m sogil. Antibacterial, antioxidant, wound healing activities and stop-bleeding effect of methanolic extract of tridax procumbens l. Leaves. Arabian journal of medicinal & aromatic plants(ajmap) v9n2 2023.
  11. Kiran prajapati, d. Singh, s.b. Mishra, p. Dubey & b. Sangameswaran. Pharmacognostical and preliminary phytochemical studies of leaves of tridax procumbens l. Ethnobotanical leaflets 12: 1283-89. Volume 2008, issue 1 2008 article 172.
  12. Catherine c. Ikewuchi, jude c. Ikewuchi*, mercy o. Ifeanacho. Phytochemical composition of tridax procumbens linn leaves: potential as a functional food. Science research publishing. Food and nutrition sciences, 2015, 6, 992-1004.
  13. Surendra agrawal , deepak mohale and g.s. Talele. Pharmacological activities of tridax procumbens (asteraceae). International journal of phytomedicines and related industries · may 2010. Medicinal plants, 2(2) may 2010.
  14. Varsharani v. Ingole  , pravin c. Mhaske  , sushma r. Katade. Phytochemistry and pharmacological aspects of tridax procumbens (l.): a systematic and comprehensive review. Phytomedicine plus 2 (2022) 100199.
  15. Nimbekar t.p., meshram a. S. Farooqui m. S. Formulation and evaluation of ointment containing natural wound healing activity of tridax procumbens. Journal of emerging technologies and innovative research (jetir) r1905m89. Jetir may 2019, volume 6, issue 5. (issn-2349-5162).
  16. Vinod gubbiveeranna, s. Nagaraju. Ethnomedicinal, phytochemical constituents and pharmacological activities of tridax procumbens: a review. International journal of pharmacy and pharmaceutical sciences. Vol 8, issue 2, 2016. Issn- 0975-1491.
  17. A.H.M.M. Rahman, M.S. Alam, S.K. Khan Ferdous Ahmed, 1 2 2 1 A.K.M. Rafiul Islam, and M. Matiur Rahman. Taxonomic Studies on the Family Asteraceae (Compositae) of the Rajshahi Division. Research Journal of Agriculture and Biological Sciences, 4(2): 134-140, 2008.
  18. Pande J, Padalia H, Donga S and Chanda S. Pharmacognostic, physicochemical and phytochemical studies of Andrographis echioides Nees. and Tridax procumbens L. leaf and stem. The Pharma Innovation Journal 2018; 7(6): 303-315.
  19. Arnab Bera  and Nilanjana Banerjee2, Physicochemical and antistaphylococcal evaluation of two herbal ointments from Mikania micrantha Kunth and Tridax procumbens Linn. Pharmacognosy Journal, Vol 15, Issue 6, Nov-Dec, 2023.

Reference

  1. Anil saini & dr.parvesh gupta. Phytochemical studies of the leaves of Tridax procumbens. International journal of research and analytical reviews. [Volume 5 i issue 3 i July– sept 2018]. E issn 2348 –1269, print issn 2349-5138.
  2. Minal Wani, Snehal Pande and Nitin More. Callus induction studies in Tridax procumbens L. International Journal of Biotechnology Applications, ISSN: 0975–2943, Volume 2, Issue 1, 2010, pp-11-14.
  3. Vikram mahadev shingade and nisha nitish karandikar. A review on pharmacological &biological activity of tridax procumbens linn (bhringraj). World journal of pharmaceutical research. Volume 10, issue 6, 705-715. Issn 2277– 7105.
  4. Asad syed  , natarajan benit  , abdullah  alyousef  , abdulaziz alqasim , mohammed arshad. In-vitro antibacterial, antioxidant potentials and cytotoxic activity of the leaves of tridax procumbens. A. Syed et al. / saudi journal of biological sciences 27 (2020) 757–761.
  5. Awasthi , irshad m, das m.k., ganti s.s , moshahid  a. Rizvi. Anti-inflammatory activity of calotropis gigantea and tridax procumbens on carrageenin-induced paw edema in rats. Ethnobotanical leaflets 13:568-77, 2009.
  6. A. Shanmugapriya and s. Maneemegalai. Phytochemical screening, antimicrobial and antioxidant activity of leaf extracts of tridax procumbens. International journal of research in pharmacy and chemistry (ijrpc) 2017, 7(3), 320-326. Issn: 2231?2781.
  7. Valivittan, k and p. Dhasarathan. Screening of phytochemicals and antimicrobial activity in tridox procumbens. Rising research journal publication.global j. Of mod. Biol. & tech., 2014: 4 (1). Issn 2277-6656 (online): 2231-5179.
  8. Durga devi.m, banu.n. Study of antioxidant activity of chlorophyll from some medicinal plants. Indian journal of research. Volume: 4 | issue : 2 | feb 2015. Issn - 2250-1991.
  9. Ashutosh bangale, dr. Sangeeta tanavade, akash bhosale, abhishek bagadi, priyanka bandichhode. Phytochemical analysis & antiinflammatoryactivity of tridax procumbens l. International journal of creative research thoughts (ijcrt)2308025. © 2023 ijcrt | volume 11, issue 8 august 2023 | issn: 2320-2882.
  10. Ammar maali , methaq n algabr , najeeb yl al-surmi , aziza m taj aldeen , hussein m sogil. Antibacterial, antioxidant, wound healing activities and stop-bleeding effect of methanolic extract of tridax procumbens l. Leaves. Arabian journal of medicinal & aromatic plants(ajmap) v9n2 2023.
  11. Kiran prajapati, d. Singh, s.b. Mishra, p. Dubey & b. Sangameswaran. Pharmacognostical and preliminary phytochemical studies of leaves of tridax procumbens l. Ethnobotanical leaflets 12: 1283-89. Volume 2008, issue 1 2008 article 172.
  12. Catherine c. Ikewuchi, jude c. Ikewuchi*, mercy o. Ifeanacho. Phytochemical composition of tridax procumbens linn leaves: potential as a functional food. Science research publishing. Food and nutrition sciences, 2015, 6, 992-1004.
  13. Surendra agrawal , deepak mohale and g.s. Talele. Pharmacological activities of tridax procumbens (asteraceae). International journal of phytomedicines and related industries · may 2010. Medicinal plants, 2(2) may 2010.
  14. Varsharani v. Ingole  , pravin c. Mhaske  , sushma r. Katade. Phytochemistry and pharmacological aspects of tridax procumbens (l.): a systematic and comprehensive review. Phytomedicine plus 2 (2022) 100199.
  15. Nimbekar t.p., meshram a. S. Farooqui m. S. Formulation and evaluation of ointment containing natural wound healing activity of tridax procumbens. Journal of emerging technologies and innovative research (jetir) r1905m89. Jetir may 2019, volume 6, issue 5. (issn-2349-5162).
  16. Vinod gubbiveeranna, s. Nagaraju. Ethnomedicinal, phytochemical constituents and pharmacological activities of tridax procumbens: a review. International journal of pharmacy and pharmaceutical sciences. Vol 8, issue 2, 2016. Issn- 0975-1491.
  17. A.H.M.M. Rahman, M.S. Alam, S.K. Khan Ferdous Ahmed, 1 2 2 1 A.K.M. Rafiul Islam, and M. Matiur Rahman. Taxonomic Studies on the Family Asteraceae (Compositae) of the Rajshahi Division. Research Journal of Agriculture and Biological Sciences, 4(2): 134-140, 2008.
  18. Pande J, Padalia H, Donga S and Chanda S. Pharmacognostic, physicochemical and phytochemical studies of Andrographis echioides Nees. and Tridax procumbens L. leaf and stem. The Pharma Innovation Journal 2018; 7(6): 303-315.
  19. Arnab Bera  and Nilanjana Banerjee2, Physicochemical and antistaphylococcal evaluation of two herbal ointments from Mikania micrantha Kunth and Tridax procumbens Linn. Pharmacognosy Journal, Vol 15, Issue 6, Nov-Dec, 2023.

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Akash Bhagwan Aher
Corresponding author

Akash Bhagwan Aher

Aher Akash Bhagwan, Phytochemical And Pharmacological Activity Of Tridax Procumbems Linn, Int. J. of Pharm. Sci., 2024, Vol 2, Issue 5, 1691-1697. https://doi.org/10.5281/zenodo.11386793

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