Human beings have depended on nature for their simple requirements, using it as a source for medicines, shelters, foodstuffs, fragrances, clothing, flavors, fertilizers, and means of transportation throughout the ages.(1) An elixir is a sweet liquid used for medical purposes, to be taken orally and intended to cure one’s illness. When used as a pharmaceutical preparation, an elixir contains at least one active ingredient and is intended for oral administration. (2) Hemoglobin deficiency is one of the most common disorders affecting mankind. Iron is needed to form Hemoglobin & mostly iron stored in body in the form of Hemoglobin (3). Iron deficiency anemia greatly affects human health, leading to symptoms like fatigue, weakness, and shortness of breath, which can diminish quality of life and productivity.

Haemoglobin Enhancer activity of plant:

These extracts are often used in herbal formulations aimed at improving blood health, particularly in conditions like anemia where hemoglobin levels are low. However, it’s essential to consult with a healthcare provider before using them, especially in therapeutic doses or if you have underlying health conditions.

MATERIAL METHOD:

Collection of plants material -

The plants required for the research like beetroot, Papaya, Lemon are collected from local shop and some from the farm.  Liquorice roots taken from college laboratory.

Preparation of Extract by using Maceration Method:

Choose the herbs we want to use based on the desired properties of the elixir. Chop fresh herbs or crush dried herbs to increase the surface area for extraction. The plant material was air-dried at room temperature and then ground into powder. Place the herbs in a clean glass jar. Fill it about halfway with herbs. Pour the solvent over the herbs until they are completely covered. Ensure there is about an inch of solvent above the herbs. Extraction of plant material by maceration method. Maceration is an extraction procedure in which coarsely powdered drug material, either leaves or stem bark or root bark, is placed inside a container; the menstruum is poured on top until completely covered the drug material. The container is then closed and kept for at least three day (4,5).

Method of Preparation:

1. Step 1 – Preparing the active drug and vehicle:

Weight accurately of drug and excipients.

Extract Carica papaya using a Soxhlet apparatus. Accurately weigh the drug and excipients. This extraction is conducted using ethanol as the solvent. The liquorice is soluble in water; dissolve it in water while continuously stirring.

2.  Step 2 – Mixing the solution:

The alcohol-soluble ingredients dissolve in alcohol, while the water-soluble ingredients dissolve in water, and then mixed them together.

3. Step 3 –Addition of preservatives and Flavoring agent and coloring agent:

The preservatives such as citric acid, methyl paraben, and propyl paraben, along with the flavoring agent liquorice and lemon juice, are used. Beet extract, containing betalain pigment, serves as the coloring agent.

4. Step 4 –Preparation of final elixir:

All ingredients are combined in a beaker and stirred continuously using a magnetic stirrer to ensure thorough mixing in the solution. Elixirs must have a crystal-clear appearance, so they should be strained and filtered as needed, and possibly clarified further if required. Transfer this solution into a measuring cylinder first, and then pour it into an amber-colored bottle or a container resistant to light (6)

Formulation Table: