Pataldhamal Wadhwani college of pharmacy, Yavatmal
The objective of this study was to develop and evaluate a floating gastroretentive in situ gel system containing Dexlansoprazole, a proton pump inhibitor used in the treatment of gastroesophageal reflux disease (GERD). Dexlansoprazole exhibits pH-dependent solubility and is primarily absorbed in the upper gastrointestinal tract, making it a suitable candidate for gastroretentive delivery. The in situ gelling system was formulated using sodium alginate in combination with pH independent polymers such as Eudragit RSPO and calcium carbonate as a cross-linking and gas-generating agent. The gels were designed to remain buoyant and undergo sol-gel transition upon contact with gastric fluid, thereby prolonging gastric residence time and improving drug bioavailability. The formulations were evaluated for viscosity, pH, gelation capacity, floating lag time, total floating duration, drug content, in vitro drug release. The optimized formulation exhibited satisfactory gelation, prolonged buoyancy (>12 hours), and sustained drug release over 8 hours following zero-order kinetics. The study concludes that floating in situ gel systems offer a promising approach for enhancing the gastric retention and therapeutic efficacy of Dexlansoprazole.
Oral route for the delivery of the medication is considered as one of the oldest and most convenient method. With the new innovations and novelty in this method has made it possible to deliver the medication in a more sustained and controlled manner. GRDDS is the desirable approach for the optimized therapeutic benefit for a drug with narrow absorption window. Gastroretentive Drug Delivery System is a Novel Drug Delivery which is mainly comprised of Floating, Mucoadhesive, Swellable and High Density system. Floating drug delivery systems was first described by Davis in 1968. These are low-density systems that have sufficient buoyancy to float over the gastric contents and remain in the stomach for a prolonged period and one of the important approaches to achieve gastric retention and to obtain sufficient drug bioavailability. This system is desirable for drugs with low absorption window in the stomach or in the upper small intestine. FDDS having a bulk density lower than gastric fluids and thus, that remain buoyant in the stomach without affecting the gastric emptying rate for a prolonged period of time.
Dexlansoprazole is a proton pump inhibitor (PPI) used primarily for the treatment of gastroesophageal reflux disease (GERD) and other conditions involving excessive stomach acid. It is the R-enantiomer of lansoprazole which provides extended acid suppression.
The present study aims to formulate and evaluate floating gastroretentive drug delivery system to enhance its gastric residance time and to increase its bioavailibility. The insitu gel system is prepared using appropriate polymers through a pH triggered method and evaluated for its physicochemical properties, drug release behavior, and floating duration.
AIM, OBJECTIVE AND NEED OF STUDY :
Aim:Formulation and Evaluation of Floating Gastroretentive Drug Delivery System .
Objective :
The main objective of present study are as below.
1.To formulate various batches of Gastroretentive floating drug delivery system by using various polymers.
2. To study the effect of polymer concentration on rate of release of drug from floating drug delivery system.
3.To study the effect of polymers and other excipients on floating lag time and total floating time .
4.To increase gastric residence time of dosage form in the stomach.
5.To reduce dosing frequency.
6.To enhance oral bioavailability of drug .
DRUG PROFILE
1. IUPAC Name: (R)-2-([3-methyl-4-(2,2,2-trifluoroethoxy)pyridin-2-yl]methylsulfinyl)-1H-benzimidazole
2. Mechanism of Action
Dexlansoprazole selectively inhibits the H+/K+ ATPase enzyme system (proton pump) in the gastric parietal cells. This inhibition blocks the final step of acid production, thereby reducing gastric acidity. The dual delayed-release system allows for two separate releases of the drug, resulting in prolonged plasma concentration and acid suppression.
3. Pharmacokinetics
4. Dosage & Administration
5. Advantages of Dexlansoprazole
6. Adverse Effects:
EXCIPIENTS PROFILE:
Table no:1 Excipients profile
INGREDIENTSNGREDIENTS |
UUSES USE |
Dexlansoprazole |
Proton pump inhibitor |
Sodium alginate |
Gelling agent |
Eudragit RSPO |
pH- independant and non biodegradable polymer |
Eudragit RLPO |
pH-indepented and stabilizer |
Xanthum gum |
Viscocity enhancer |
Guar gum |
Viscocity increasing polymer |
Sodium bicarbonate |
Buoyancy enhancer |
Calcium carbonate |
Gas forming agent |
Calcium chloride |
Cross linking agent |
Sodium citrate |
As a preservative or maintain the fluidity of formulation |
Distilled water |
As a vehicle |
MATERIALS AND METHODS:
1. Analytical chacterization of drug sample:
Stock solution (100μg/ml) of dexlansoprazole was prepared in 0.1 HCL. This solution was appropriately diluted with 0.1 N HCl separately to obtain a concentration of 100 µg/ml dexlansoprazole in 0.1 N HCl and water respectively. The UV spectrum was recorded in the range of 200-400 nm on UV Visible Spectrophotometer.
2. FT-IR SPECTROSCOPY:
The FTIR studies are performed to observe any interaction between drug and polymers in the formulation. FTIR study of optimized floating insitu gel(F6 batch) was carried out. The FTIR spectra indicate that there is no interaction betweensodium alginate & drug within insitu gel.
The spectrum of optimized floating insitu gel was found to be similar to pure dexlansoprazole drug.FTIR spectra shoes key functional peak of dexlansoprazole are retained in physical mixture spectrum such as C=C,C=O,C-H that confirms integrity of drug structure .There are additional broad peak such as at 3246.79cm-1 due to hydroxyl group from sodium alginate and shift around 1749.44 cm-1 likely due to Eudragit RSPO ester carbonyl. These donot overlap or mask drug peak, suggesting no major chemical interaction has occured.
3. Prepartion of floating insitu gel:
The formulation were prepared as given in table by heating polymer at 600C in deionized water with continious stirring .After cooling below 400C gas forming agent Calcium carbonate ,cross linking agent Calcium chloride ,buoyancy enhancer Sodium carbonate ,Dexlansoprazole were added with continious stirring.
Table no 2: Prepartion of floating insitu gel
Ingredients
|
F1 |
F2 |
F3 |
F4 |
F5 |
F6 |
F7 |
F8 |
F9 |
F10 |
Dexlansoprazole (mg)
|
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
Sodium alginate(%) |
0.5 |
1 |
1.5 |
1.5 |
2 |
2 |
2 |
2 |
2 |
2 |
Eudragit RSPO(%) |
|
- |
|
1.5 |
- |
2 |
- |
- |
- |
- |
Eudragit RLPO(%) |
- |
- |
- |
- |
- |
- |
1 |
2 |
- |
- |
Xanthum gum(%) |
- |
- |
- |
- |
- |
- |
- |
- |
0.5 |
- |
Guar gum(%) |
- |
- |
- |
- |
- |
- |
- |
- |
- |
0.5 |
Calcium carbonate(%) |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
Sodium bicarbonate(%) |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
Calcium chloride(%) |
0.15 |
0.15 |
0.15 |
0.15 |
0.15 |
0.15 |
0.15 |
0.15 |
0.15 |
0.15 |
Sodium citrate(%) |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
Distilled water(ml) |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
RESULTS AND DISCUSSION
Preformulation studies
Physical Characterization of Dexlansoprazole:
The recived sample of Dexlansoprazole was found to show the following characteristics.
Nature: Pale yellowish color
Melting point :1390C-1400C
Solubility: highly soluble in water, methanol, ethanol.
Identification of drug:
Standard calibration curve of Dexlansoprazole at 285 nm follows Beer’s -Lamberts Law in the concentration range 2-10 mcg /ml.
Table no.3. Absorbance of Dexlansoprazole in 0.1N HCl
Concentration (μg/ml) |
Absorbance |
0 |
0 |
2 |
0.045 |
4 |
0.107 |
6 |
0.153 |
8 |
0.198 |
10 |
0.250 |
Fig no. 1. Calibration curve of Dexlansoprazole in 0.1 N HCl
Drug excipient interaction plays a vital role in the release of drug from formulation .The pure drug Dexlansoprazole and its physical mixture with sodium alginate and Eudragit RSPO were scanned using FTIR instrument .Here we observed that key functional peak of dexlansoprazole are retained in physical mixture spectrum such as C=C,C=O,C-H that confirms integrity of drug structure .There are additional broad peak such as at 3246.79cm-1 due to hydroxyl group from sodium alginate and shift around 1749.44 cm-1 likely due to Eudragit RSPO ester carbonyl. These donot overlap or mask drug peak, suggesting no major chemical interaction has occured.
Fig no2. FTIR of Dexlansoprazole
Fig no3. FTIR of drug and polymer
Physicochemical characteristics of formulated insitu gel:
Table no. 4. Physico-chemical studies
Batches
|
pH |
Viscocity |
Gelling time |
Floating lag time(sec) |
Floating duration (hr) |
Drug content (%) |
F1 |
- |
- |
- |
Does not float |
- |
- |
F2 |
- |
- |
- |
Does not float |
- |
- |
F3 |
9.3 |
5 |
4-5 |
4-5 |
>12 |
71.26% |
F4 |
9.7 |
7.41 |
3-4 |
4-5 |
>12 |
85% |
F5 |
9.8 |
6.1 |
2-3 |
3-4 |
>12 |
91.84% |
F6 |
10.2 |
9.58 |
immediate |
immediate |
>12 |
93.75% |
F7 |
8.94 |
8.97 |
4-5 |
2-3 |
>12 |
74.3% |
F8 |
8.92 |
9.06 |
4-5 |
2-3 |
>12 |
80.82% |
F9 |
8.4 |
4.37 |
6-7 |
6-7 |
>12 |
72.8% |
F10 |
9.7 |
7.81 |
6-7 |
10 |
>12 |
60% |
The pH of each formulation was measured using a calibrated digital pH meter at 270C. The pH of all formulation was found to be within range of 8.4-10.2. It was found that the optimized batche F6 showed 10.2 pH respectively.
The viscosity of all formulation was determined by Brrokfield viscometer DV-II( Brrokfield,USA) using spindle number 62 with cuo and bob setting at 30 rpm .It was found that the optimized batches F6 showed 9.58 cps viscosity respectively.
The gelling capacity of prepared formulation was observed by visual examination.All the prepared batches show gelling time 2-6 sec to immediate after entering in 0.1N HCl .The optimized batch show gelling time 1-2 sec after getting contact wit 0.1 N HCl and remain in the form of gel for upto more than 12 hrs.
Floating lag time of all the prepared formulations were observed by visual examination. AlI prepared formulations show floating lag time 2 sec -10 sec and the optimized batch show 1-2 sec floating lag time after entering in 0.1 N HCl and show floating for more than 12 hr respectively.
Floating duration time of all the prepared formulations was observed by visual examination. All the prepared formulations show floating duration time from 12 to 24 hrs. The optimized batch F6 showed more than 12 hrs respectively.
The optimized batch F6 showed 93.75% respectively.
The dug release study was performed using USP Type II dissolution test apparatus in 0.1N HCI buffer of with pH 1.2. The temperature and speed of the apparatus were maintained at 37oC and 50 rpm respectively. The samples were withdrawn at predetemined time interval and analyzed for drug concentration at 285 nm by UV-Visible
Table no.5. Invitro drug release
Time in hr |
F1 |
F2 |
F3 |
F4 |
F5 |
F6 |
F7 |
F8 |
F9 |
F10 |
1 |
- |
- |
17.11% |
21.41% |
19.05% |
17.11% |
40.44% |
26.83% |
41% |
42.6% |
2 |
- |
- |
34.6% |
38.6% |
26.18% |
24.35% |
46.27% |
32.66% |
57.28% |
58.2% |
3 |
- |
- |
51.45% |
51.94% |
43.68% |
26.83% |
49.51% |
39.79% |
61.82% |
64.85% |
4 |
- |
- |
79.32% |
68.76% |
54.69% |
43.68% |
63.76% |
48.21% |
92.28% |
87.74% |
5 |
- |
- |
89.68% |
79.8% |
63.76% |
54.69% |
78.02% |
52.10% |
97.46% |
92.4% |
6 |
- |
- |
93.57% |
82.5% |
82.56% |
63.76% |
86.44% |
79.96% |
|
|
7 |
- |
- |
|
87.24% |
92.22% |
79.8% |
97.33% |
86.28% |
|
|
8 |
- |
- |
|
94.2% |
94.35% |
82.85% |
|
95.20% |
|
|
9 |
- |
- |
|
|
98.20% |
87.09% |
|
|
|
|
Fig no. 4. Invitro drug release graph
SUMMARY AND CONCLUSION
From the result it can be concluded that the formulated dexlansoprazole insitu gel was found to be easier, simpler and sucessfully produced pH triggered in-situ gel. It was found F6 batch is optimized batch having drug release 87.09% for 9 hrs which have better floating efficacy and provided sustained invitro release profile over an extented period. Hence it may represent as a new alternavtive biodegradable and cheaper formulation which may provide improve the patient compliance, comfort and sustained release.
REFERENCES
Mayuri Raut, Dr. A. Chandewar, Dr. Madhuri Channawar, Payal Malekar, Formulation And Evaluation of Floating Gastroretentive Drug and Delivery System, Int. J. of Pharm. Sci., 2025, Vol 3, Issue 5, 2878-2887. https://doi.org/10.5281/zenodo.15449809