1,2Oriental University, Indore.
3Shri Bherulal Pharmacy Institute, Indore.
4Shri Sahaj Institute of Pharmacy, Khargone.
5Swami Vivekanand College of Pharmacy.
6Patel College of Pharmacy.
The current goal is to create a topical Polyherbal suspension that contains guggul to treat anti-inflammatory activities. Several physicochemical characteristics of the developed Polyherbal suspension were assessed. Inflammation is typically created by a combination of the innate immune system and the adaptive immune system. The innate immune system, which includes the activity of different cells like macrophages, mast cells, and dendritic cells, is the body's main line of defense against invasive microorganisms and cancer cells. suspensions are a significant subcategory of pharmaceutical formulations and pose numerous difficulties to formula development personnel.it is of two types flocculated and Non flocculated, Suspension formulated with Guggul (commiphora wightii) is used to treat the Inflammation or has the Anti-inflammatory activity, Guggul is an oleo gum-resin& it's composition is made up of resin, gum, volatile oils, moisture, and foreign matter. The formulated suspension was created with a particle size analysis that falls between 15µm and 18µm. Utilizing a 5.5 PH phosphate buffer and dimethyl sulfoxide solution; the in vitro dffusion release was examined. Utilizing various kinetic models, including zero order, first order, higuchi plot, peppas plot, and hixon plot models, the drug release mechanism of these formulations has been described and it follows the Non fickan mechanism of transport with release mechanism of both diffusion and relaxation. Invivo studies were also performed using the rat and the inflammation was seen reducing. The formula F4 Compared to other formulations showed the results satisfactory. I discussed the Polyherbal suspension in this paper.It also discusses the anti-inflammatory activity and contains benefits, drawbacks, methodology, evaluation, kinetic release, and results.
Traditional system of medicine
Traditional medical systems have long played a significant role in addressing the demands of the world's population in terms of healthcare. Currently, they are doing so, and they will continue to play a significant role in the future. The term "Indian Systems of Medicine" refers to either the medical systems that are believed to have originated in India or the systems that were introduced to India from elsewhere and integrated into Indian culture. Six internationally recognized medical systems are found in India, which is a unique distinction. The following are among them: Ayurveda, Siddha, Unani, Yoga, Naturopathy, and Homoeopathy [1].
History of Polyherbal medicine
Mankind has always looked for ways to treat illness and relieve pain. There is evidence of the use of medicinal plants dating back 60,000 years, but more recently, a 5000-year-old clay slab from Sumerian culture was found to confirm the use of medicinal plants for drug preparation. More than 50% of currently available drugs are thought to have some connection to plants. Theae folium, Rhei rhizoma, camphor, podophyllum, jimson weed, and ephedra are just a few of the 365 drugs listed in the Chinese book "Pen Ts'ao" by Emperor Shen Nung [2].
Anti-inflammatory activity
When bacterial, viral, or fungal pathogens enter the body, settle in specific tissues, or circulate in the blood, inflammation frequently results. As a result of conditions like tissue damage, cell death, cancer, ischemia, and degeneration, inflammation may also develop. Inflammation is typically created by a combination of the innate immune system and the adaptive immune system[3].
Process of Inflammation
The four cardinal signs of inflammation are heat, redness, pain, and swelling. The blood vessels' intercellular spaces and increased blood flow are made possible by this, which causes "leaky vessels." Consequently, the injured area can receive fluids, leukocytes, and plasma proteins to help with inflammation. The release of chemical messengers known as chemokine’s by mast cells and injured tissue cells is another crucial mechanism. These substances, also known as chemo attractants, produce a chemical gradient that leukocytes travel along in order to get to the inflamed area. These chemicals also have other effects, such as producing heat, enhancing enzyme activity, causing pain,and increasing vascular permeability. Leukotriene’s, cytokines, histamine, prostaglandins, and others are a few examples of these chemokine’s[4].
Consequences of Inflammation:-Heat at the injury's site, Blotchy skin, Swelling, Tenderness, The signs of chronic inflammation are more subtle than those of acute inflammation, Chest discomfort, Continent pain, Fever, tuberculosis, Mouth ulcers Pain or stiffness in the joints, skin rash, such as psoriasis, Exhaustion[5].
Classification: -Generally speaking, there are two types of inflammation: acute and chronic [6].
Table1: - Classification of Suspension
|
|
Acute |
Chronic |
|
Cause |
Harmful pathogens or tissue injury. |
viruses, persistent foreign bodies, excessive immune reactions, and other pathogens that the body is unable to eliminate. |
|
Initiation |
Rapid |
Slow |
|
Duration of action |
Few days |
Months to Years |
|
Result/outcome |
Inflammation improves, oranabsces develops or becomes chronic. |
Connective tissue scarring, tissue death and tissue thickening. |
Inflammation treatment
Usually no treatment or medication is needed for inflammation. For instance, adequate rest, ice application, and proper wound care can all be used to treat the symptoms of acute inflammation. However, depending on your condition and the severity of your chronic inflammation, your doctor may advise one of the following treatments [7] Supplements, NSAIDs, Steroid injections, the swelling and pain brought on by a variety of illnesses and injuries can be lessened with topical anti-inflammatory creams. Common topical anti- inflammatory drugs include the gel form of Voltaren (diclofenac), capsaicin cream, and menthol cream [8].
Suspension
Due to their inherent structural instability, issues with manufacturing, and issues with packaging, suspensions are a significant subcategory of pharmaceutical formulations and pose numerous difficulties to formula development personnel. It depends on the suspension whether it is intended for parenteral use, external application, or oral administration.[9,10] They typically consist of a finely divided solid suspended in a liquid or semi-solid medium, which is the continuous phase. Individual particles in these mixtures typically range in size from 0.5 to 5.0 m. nowadays, many suspensions are sold as dry powders that must be "constituted" before use by adding a vehicle in the prescribed amounts. Such "suspensions" are created primarily for stability-related reasons [11].
Polyherbal Suspension:-Suspension that is formulated with the Polyherbal ingredients is Polyherbal suspension. Suspension formulated with Guggul (comiphora wightii) is used to treat the Inflammation or has the Anti-inflammatory activity [12].
2.MATERIAL & METHOD:
Pre- Formulation studies
Pre-formulation investigations are designed to deliver all necessary data (physicochemical characteristics of drug and excipients) which may influence the following:- Formulation Design, Method of manufacturing of the drug product, Evaluations of the resulting product, Packaging of the product[13,14].
Solubility studies of the drug (guggul)
Procedure: - solubility studies of the drug was done using different selected media or solvents, It was done by dissolving the known quantity of drug in a cumulative manner until they remain insoluble in the media[15].The solvents used for dissolving are as follows:
Determination of (λmax)
UV Scan range of 200 to 800 nm was selected to determine maximum absorbance of guggul and alma by using 10 µg/ml solutions the wavelength corresponding to maximum absorbance was found to be 270 nm for guggul and found at 385nm of alma respectively. The λmax values are shown [18].
Determination of Calibration curve Preparation of Standard Solution:
A Stock solution of guggul is prepared by dissolving 100mg pure drug in 10ml DMSO made upto 100 ml with distilled water. From the above solution take 10 ml and diluted to 100ml with distilled water [19,20].
Linearity
Different aliquots of working standard of guggul ranges from 1-10 µg/ml was transferred into series of volumetric flask and total volume was made up to 10 ml with solvent the absorbance was measured at 270 nm against the blank. The linearity is show [21].
MATERIALS AND METHOD: -
Materials: -guggul is taken from the Yucca Enterprises, amla, CMC, Turmericis also from Yucca Enterprises and distilled water from central laboratory.
Table1: -Formulation Development
|
Ingredients |
F1 |
F2 |
F3 |
F4 |
F5 |
F6 |
F7 |
F8 |
F9 |
|
Guggul(g) |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
|
Alma(g) |
1.5 |
2 |
2.5 |
2 |
2 |
2 |
2 |
1.5 |
2.5 |
|
Carboxymethyl cellulose(g) |
3 |
1.5 |
2 |
3 |
2.5 |
3 |
3.5 |
3.5 |
3.5 |
|
Turmeric(g) |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
|
Distilled water(ml) |
Q. S |
Q. S |
Q. S |
Q. S |
Q. S |
Q. S |
Q. S |
Q. S |
Q. S |
Methodology (Procedure):
Required quantity of drug and excipients was weighed and passed through the sieve number 100, Now the drug is added to the mortar and pestle and triturate well then add the Amla powder and triturate well, then add the Carboxy methyl cellulose followed by the addition of turmeric powder, Now add the distilled water drop wise and triturate well, finally make up the volume to 100 ml with distilled water.
Evaluation of Suspension
pH: The hydrogen ion concentration's negative logarithm is known as pH. The formula is pH=log 1/(H3O+) in mathematics. pH=-log(H3O+) because the logarithm of 1 is equal to zero. A pH metre was used to determine the suspension's pH [22, 23].
Viscosity: -When the solid fraction is higher, the suspension viscosity is higher; when the average particle size is higher, it is lower. For the dependence of the relative viscosity on the solid fraction, many theoretical and semi-empirical closed-form relations have been proposed [24].
Sedimentation method:- To calculate sedimentation, two parameters are studied: Volume of sedimentation, Flocculation level[25].
Rheological method:-It offers details on the habits of Settlers. The viscosity of the suspension is investigated using a Brookfield viscometer. It is fixed with a T-bar spindle and heli path stand. The resistance the T-bar spindle encounters at different levels is then measured by the viscometer's dial reading as the spindle gently descends into the suspension. This method also identifies the level of the suspension where the structure is stronger as a result of particle agglomeration. The dial reading is plotted against the spindle's number of rotations. The better suspension exhibits a slower rate of dial reading amplification with spindle spins, so the curve is horizontal for an extended period of time [26, 27].
Microbial testing of finished product:-This test is typically carried out to determine whether or not the finished product has any presence of microorganisms.
Two media were used for this test in a common fashion:
Estimating Stability
Finished Product Stability Research
Drug stability is the period of time from the date of manufacture and packaging of the formulation until its chemical or biological activity is not less than a set level of labeled potency and its physical features have not changed noticeably or negatively. The length of the study and the storage conditions are outlined by ICH. Long-term testing for 12 months at 25°C, 2°C, 60% RH, and 5%Rapid testing at 40°C, 2°C, and 75% relative humidity for six months [29,30].
Diffusion Studies:-
In vitro permeation in egg membrane: -
In vitro diffusion studies for all formulations were carried out using Franz diffusion cells. The cell is fabricated with 5mm amber unjacketed and 3ml receptor volume.10 ml 0f DMSO (dimethyl sulfoxide) is made up to 100 ml with 5.5 pH phosphate buffer and this is used as the receptor medium. Egg membrane is used as the dialysis membrane; the membrane is tied to the diffusion cell (donar). Receptor media was added to a donor compartment prior to being mounted on a diffusion cell, A measured quantity of formulation equivalent to 1ml was taken on to the egg membrane and immersed slightly in the receptor medium. [31,32] The entire system was maintained at 37 +|-1oc. An Aliquot of 1 ml was drawn at specific time intervals for 8 hrs. and made up the volume to 10 ml with buffer solution. It was estimated Spectrometrically at 270 nm after each withdrawal the diffusion medium is replaced with an equal volume of fresh medium and % Cumulative release was calculated [33].
Invivo studies:
The average weight of the rat cohort was 180 ± 10 g. The rats were retained in standard laboratory conditions (22 ± 2 °C room temperature and 40–50% relative humidity) with 12 h light and dark cycles, and were fed and watered. Prior to the experiment, the animals were fed pellet food and acclimatized to laboratory conditions for 2 days. Initially plethysmometer was filled with mercury up to the scale reading zero, then rat left paw was dip in the mercury and recorded the readings [34,35]. Now 0.1 mL saline solution of 1%w/v carrageenan of about (0.06ml) was injected to the left paw and left for about 30 mins.then inflammation was observed. A quantity of 5 ml of the test guggul Polyherbal suspension formulation was spread uniformly by massaging repeatedly for 50 times in order to facilitate absorption through the skin of the animal’s paw. Leave the animal for 30 min and inflammation was observed for 6hrs (0min, 30min, 1hr, 2hrs, 4hrs, 6hrs) at periodic intervals of time [36].
RESULTS AND DISSCUSION:
Pre-formulation Studies: -Solubility studies were done for drug (guggul)
TABLE 2: - Solubility studies for Drug
|
Solvents |
Solubility |
Readings in Percentage |
|
Distilled water |
Insoluble |
0 |
|
Methanol |
Sparingly soluble |
20 |
|
Ethanol |
Sparingly soluble |
25 |
|
Chloroform |
Sparingly soluble |
30 |
|
Acetone |
Sparingly soluble |
50 |
|
Phosphate buffer |
Sparingly soluble |
60 |
|
Dimethyl Sulfoxide |
Soluble |
99 |
From the above table the active ingredient is soluble in the DMSO
Determination of Wavelength:
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<img alt="Determination of Wavelength.png" height="150" src="https://www.ijpsjournal.com/uploads/createUrl/createUrl-20250423115439-12.png" width="150">
</a>
The wave length was found to be 270nm for the active ingredient.
FTIR Studies
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<img alt="FTIR Studies of Guggul.png" height="150" src="https://www.ijpsjournal.com/uploads/createUrl/createUrl-20250423115439-11.png" width="150">
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FIG 3: - FTIR Studies of Guggul
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<img alt="FTIR Studies of F4 Formulation.png" height="150" src="https://www.ijpsjournal.com/uploads/createUrl/createUrl-20250423115439-10.png" width="150">
</a>
Fig 4: - FTIR Studies of F4 Formulation
Table 3: -Standard Calibration Curve
|
S. No |
Concentration |
Absorbance |
|
1 |
0.2 |
0.101±0.03 |
|
2 |
0.4 |
0.174±0.03 |
|
3 |
0.6 |
0.277±0.03 |
|
4 |
0.8 |
0.390±0.03 |
|
5 |
1.0 |
0.552±0.03 |
Each value represents mean values of ±s.d. (n=3)
Fig 5: -calibration curve of guggul
Evaluations:
Table 5: -
|
Formulations |
Re- Dispensability |
PH |
Nature |
Colour |
Odour |
Particle Size |
|
F1 |
Inversion |
4.2 |
Suspension |
Yellow |
Characteristic |
15µm |
|
F2 |
Inversion |
4.25 |
Suspension |
Yellow |
Characteristic |
14µm |
|
F3 |
Inversion |
5 |
Suspension |
Yellow |
Characteristic |
14µm |
|
F4 |
Inversion |
5 |
Suspension |
Yellow |
Characteristic |
14.5µm |
|
F5 |
Inversion |
5.1 |
Suspension |
Yellow |
Characteristic |
13.5µm |
|
F6 |
Inversion |
5.2 |
Suspension |
Yellow |
Characteristic |
13µm |
|
F7 |
No Inversion |
5.25 |
Cream |
Yellow |
Characteristic |
16µm |
|
F8 |
No Inversion |
5.5 |
Cream |
Yellow |
Characteristic |
16.8µm |
|
F9 |
No Inversion |
5.5 |
Cream |
Yellow |
Characteristic |
18µm |
From the above table the redispersibility, Nature studies F1to F6 shows inversion and suspension whereas F7 to F9 shows no inversion and as cream, F4 shows optimized PH, and particle size 14.5µm, all the formulations show yellow colour and characteristic odor.
Table 6: -Viscosity: -viscosity of sample was determined at room temperature by using Brookfield viscometer at 50 RPM.
|
Formulations (CP) |
F1 |
F2 |
F3 |
F4 |
F5 |
F6 |
F7 |
F8 |
F9 |
|
Spindle (61) |
48.5 |
49.2 |
51.2 |
53.2 |
46.2 |
40.0 |
38.2 |
30.1 |
26.2 |
|
Spindle (62) |
50.2 |
50.9 |
54.6 |
54.8 |
47.3 |
38.2 |
36.4 |
28.9 |
24.1 |
|
Spindle (63) |
51.8 |
51.9 |
55.6 |
56.6 |
48.4 |
38.1 |
32.1 |
26.4 |
20.2 |
|
Average value (CP) |
50.1 |
50.6 |
53.8 |
54.8 |
47.3 |
38.7 |
35.5 |
28.4 |
23.5 |
From the above table F4 showed the best result with 54.8cp.
Table 7: - Sedimentation Volume
|
Formulation |
5days |
10days |
15 days |
20 days |
25days |
30days |
|
F1 |
1.03 |
0.98 |
0.96 |
0.9 |
0.89 |
0.85 |
|
F2 |
1.02 |
0.94 |
0.9 |
0.85 |
0.82 |
0.79 |
|
F3 |
1.01 |
0.93 |
0.86 |
0.81 |
0.78 |
0.75 |
|
F4 |
1.02 |
0.97 |
0.95 |
0.93 |
0.91 |
0.88 |
|
F5 |
1.01 |
0.92 |
0.84 |
0.8 |
0.77 |
0.72 |
|
F6 |
0.97 |
0.92 |
0.89 |
0.83 |
0.76 |
0.7 |
<a href="https://www.ijpsjournal.com/uploads/createUrl/createUrl-20250423115439-9.png" target="_blank">
<img alt="Representing Sedimentation Volume.png" height="150" src="https://www.ijpsjournal.com/uploads/createUrl/createUrl-20250423115439-9.png" width="150">
</a>
Fig 6: - Representing Sedimentation Volume
Microbial Testing
Table: - Microbial Testing of Finished Product
|
S. No |
Observation |
Accelerated condition for 3 months |
Real time condition for 3 months |
Refrigeration condition for 3 months |
|
1 |
Before Incubation |
Nil |
Nil |
Nil |
|
a) Total Bacterial Count |
||||
|
b) Total fungal Count |
Nil |
Nil |
Nil |
|
|
2 |
After Incubation |
16 CFU/ml |
Nil |
Nil |
|
a) Total Bacterial Count |
||||
|
b) Total Fungal Count |
Nil |
Nil |
Nil |
Various storage setups were used to keep the optimal F4 formulation. Checks for microbiological contamination were made on the product during storage. 16 bacterial colonies were discovered to have generated under expedited settings for this formulation (F4), however they were discovered to be within acceptable bounds. Both in the accelerated and real-time situations, there were no countable fungal colonies visible.
Estimating stability
Finished product stability research
Table: Stability Studies of Formulated Polyherbal Suspension (Formulation F4)
|
S. NO |
Evaluation Test |
Accelerated conditionfor3months |
Real time condition for 3months |
|
1 |
Colour |
No change |
No change |
|
2 |
pH |
No change |
No change |
|
3 |
Viscosity |
54.8cps |
53.9cp |
|
4 |
Sedimentation volume |
0.95 |
0.95 |
Table: Stability studies of formulated Polyherbal Suspension under Refrigeration condition (Formulation F4)
|
S.NO |
Evaluation Test |
Refrigeration condition for 3 months |
|
1 |
Colour |
No change |
|
2 |
pH |
No change |
|
3 |
Viscosity |
54.8cps |
|
4 |
Sedimentation volume |
0.95 |
In Vitro Diffusion Studies:
|
Time(min) |
Cumulative % Drug Release |
|||||
|
F1 |
F2 |
F3 |
F4 |
F5 |
F6 |
|
|
0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
|
5 |
39.80±0.06 |
32.52±0.05 |
31.57±0.012 |
40.43±0.09 |
47.57±0.09 |
32.39±0.07 |
|
10 |
55.32±0.05 |
47.17±0.09 |
43.03±0.09 |
63.82±0.010 |
69.20±0.07 |
59.72±0.010 |
|
15 |
71.40±0.007 |
54.53±0.005 |
52.17±0.006 |
77.58±0.005 |
79.39±0.012 |
75.72±0.012 |
|
20 |
84.62±0.008 |
68.82±0.007 |
64.72±0.005 |
87.95±0.006 |
89.33±0.014 |
84.74±0.024 |
|
25 |
87.22±0.012 |
72.24±0.014 |
73.32±0.011 |
94.45±0.012 |
96.52±0.017 |
90.42±0.126 |
|
30 |
91.30±0.014 |
86.95±0.012 |
85.09±0.001 |
98.99±0.001 |
102.06±0.124 |
94.06±0.128 |
Each value represents mean values of ±s.d. (n=3)
Zero order drug release
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<img alt="Graph Of Zero Order.png" height="150" src="https://www.ijpsjournal.com/uploads/createUrl/createUrl-20250423115439-8.png" width="150">
</a>
Fig 10: - Graph Of Zero Order
From the above table F4 is the best formulation for the zero order First order drug release
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<img alt="Graph of for First Order.png" height="150" src="https://www.ijpsjournal.com/uploads/createUrl/createUrl-20250423115439-7.png" width="150">
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Fig 11: - Graph of for First Order
From the above table F4 is the best formulation for the first order
Higuchi Plot Time
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<img alt="Graph of Higuchi Plot Time.png" height="150" src="https://www.ijpsjournal.com/uploads/createUrl/createUrl-20250423115439-6.png" width="150">
</a>
Fig 12: - Graph of Higuchi Plot Time
From the above table F4 is the best formulation for the Higuchi plot time
Korsmeyer Peppas Plot
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<img alt="Graph of Korsmeyer Peppas Plot Time.png" height="150" src="https://www.ijpsjournal.com/uploads/createUrl/createUrl-20250423115439-5.png" width="150">
</a>
Fig 13: - Graph of Korsmeyer Peppas Plot Time
From the above table F4 is the best formulation for the Korsmeyer plot time
Hixon Crowell Plot
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<img alt="Graph Of Hixon Crowell Plot Time.png" height="150" src="https://www.ijpsjournal.com/uploads/createUrl/createUrl-20250423115439-4.png" width="150">
</a>
Fig 14: - Graph Of Hixon Crowell Plot Time
From the above table F4 is the best formulation for the Hixon plot time.
Slope and r2 Values Table 22: -
|
Formulation |
zero order |
Frist order |
Higuchi Plot |
Korsmeyer -Peppas Plot |
Hixon plot |
|||||
|
R2 |
Slope |
R2 |
Slope |
R2 |
Slope |
R2 |
Slope |
R2 |
Slope |
|
|
F1 |
0.9506 |
3.2462 |
0.2642 |
0.0843 |
0.4897 |
2.4969 |
0.9315 |
1.44 |
-2.048 |
1.3692 |
|
F2 |
0.8842 |
3.4374 |
0.194 |
0.0857 |
0.8682 |
2.2909 |
0.9141 |
1.4698 |
-1.708 |
1.178 |
|
F3 |
0.8892 |
3.5209 |
0.1737 |
0.0862 |
0.8744 |
2.0509 |
0.906 |
1.4797 |
-1.589 |
1.0945 |
|
F4 |
0.9506 |
3.6923 |
0.1596 |
0.0872 |
0.7108 |
2.3538 |
0.9012 |
1.4973 |
-1.328 |
0.9231 |
|
F5 |
0.8438 |
3.644 |
0.116 |
0.087 |
0.8155 |
2.2524 |
0.8862 |
1.4977 |
-1.406 |
0.9714 |
|
F6 |
0.8413 |
3.5516 |
0.1076 |
0.0865 |
0.8964 |
1.9614 |
0.8823 |
1.4894 |
-1.555 |
1.0637 |
In vivo studies Table 23: -
|
Time (Min) |
Level (ML) |
|
0 |
0.4 |
|
30 |
1.5 |
|
60 |
1.4 |
|
120 |
1 |
|
240 |
0.8 |
|
480 |
0.5 |
From the above table it is seen that the level of Inflammation decreases as the duration of time increases.
<a href="https://www.ijpsjournal.com/uploads/createUrl/createUrl-20250423115439-3.png" target="_blank">
<img alt="Plethysmometer.png" height="150" src="https://www.ijpsjournal.com/uploads/createUrl/createUrl-20250423115439-3.png" width="150">
</a>
Fig: -Plethysmometer
<a href="https://www.ijpsjournal.com/uploads/createUrl/createUrl-20250423115439-2.png" target="_blank">
<img alt="Rat Paw Before Inflammation.png" height="150" src="https://www.ijpsjournal.com/uploads/createUrl/createUrl-20250423115439-2.png" width="150">
</a>
Fig: - Rat Paw Before Inflammation
<a href="https://www.ijpsjournal.com/uploads/createUrl/createUrl-20250423115439-1.png" target="_blank">
<img alt="Rat Paw After Inflammation.png" height="150" src="https://www.ijpsjournal.com/uploads/createUrl/createUrl-20250423115439-1.png" width="150">
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Fig: - Rat Paw After Inflammation
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Fig: -Invivo Studies On Rat
Summary: -
The aim of the current study is to develop and evaluate the topical Polyherbal suspension using guggul as the active pharmaceutical ingredient. As per the preliminary research, it is shown that it aid in the treatment of some anti- inflammatory conditions like arthritis, psoriasis, eczema, and a variety of skin diseases like acne. Additionally, it has been utilized to treat hypothyroidism, encourage weight loss, and regulate blood sugar and cholesterol levels. But Now our study only says or discusses about the anti-inflammatory activity using various excipients which includes Amla, Carboxy methyl cellulose and Turmeric we used mostly all the Polyherbal ingredients so there will be the minimal side effects, and all these materials are available at low cost in the market. from the study it is shown that the formulated Polyherbal suspension reduces the inflammation. Guggul Polyherbal suspension was prepared by using guggul as drug and Amla, CMC, Turmeric as excipients. The physicochemical characteristics of the formulations were studied the particle size range of the prepared formulation lies from 15µm to 18µm.Redispersibility studies were performed were F1 to F6 shows inversion and F7 to F9 doesn’t show inversion. Viscosity studies were performed by using three spindles (61,62,63) and F4 formulation showed the best result of 54.8%. Sedimentation parameters are also performed from 5 days to 30 days were F4 formulation showed the best result. The microbial studies were performed for F4 formulation and 16 bacterial colonies were observed Invitro diffusion studies were studied using the egg membrane as a diffusive membrane were F4 formulation showed the best result of 97.99%.Invivo studies were performed using the rat and the result was found to be satisfactory as the inflammation is seen reducing. Finally F4 formulation is considered to be the best formulation among all the studies as it showed the optimum results.
CONCLUSION: -
Guggul Polyherbal suspension for anti-inflammatory activity was prepared by using different excipients (Amla, Carboxy methyl cellulose and Turmeric) it was concluded that the formulation F4 showed the satisfactory results compared to other formulations of F1,F2,F3,F5,F6,F7,F8,F9.
REFERENCES
Osheen Gurung, Rashmi Mishra*, Deepak Shrivastava, Ankit Sharma, Rajat Pawar, Shivam Soni, Development and Evaluation of Topical Polyherbal Suspension for Treatment of Inflammation, Int. J. of Pharm. Sci., 2025, Vol 3, Issue 4, 2732-2746 https://doi.org/10.5281/zenodo.15266622
10.5281/zenodo.15266622
