1D. Y. Patil College of Pharmacy (Deemed to Be University) Kolhapur.
2Gajanan Maharaj college of Pharmacy Nipani.
3OJAS college of pharmacy Jalna.
4Aditya Pharmacy College Beed.
5Dr J. J. Magdum Pharmacy College Jaysingpur.
Background: The increasing demand for natural cosmetic products has driven research into plant-based alternatives to synthetic formulations. Vanishing creams are oil-in-water (O/W) emulsions widely used for their non-greasy texture and skin-smoothing properties. Synthetic creams, though effective, often lead to undesirable skin reactions. In this study, an herbal vanishing cream was formulated using an ethanolic extract of Daucus carota (carrot), a plant known for its rich content of ?-carotene, vitamins A, K, B6, and antioxidants. Objective: To design, develop, and evaluate a natural vanishing cream incorporating the ethanolic taproot extract of Daucus carota with moisturizing, anti-aging, and non-irritant properties. Methods: Fresh carrots were subjected to Soxhlet extraction using ethanol as a solvent. The extract was then screened for phytochemicals, revealing the presence of alkaloids, carbohydrates, flavonoids, tannins, proteins, and phenolic compounds. The vanishing cream was prepared via melt emulsification using standard cosmetic excipients. The formulation was evaluated for pH, Spreadability, viscosity, homogeneity, emulsion type, extrudability, and skin irritancy. Results: The cream exhibited favorable physicochemical characteristics including pH (6.7–6.98), Spreadability (11.30–13.33 g·cm/sec), and viscosity (~5250 cps). No signs of erythema, oedema, or skin irritation were observed in a 72-hour human patch test. The cream remained stable with no phase separation or texture alteration at room temperature. Phytochemical constituents present in Daucus carota contributed to its antioxidant, anti-wrinkle, and moisturizing effects. Conclusion: The formulated herbal vanishing cream showed excellent stability, cosmetic appeal, and skin compatibility. Rich in natural bioactives, it offers a safe and effective alternative to synthetic creams. Further studies are warranted to explore its long-term efficacy and consumer acceptability.
Background
The contemporary cosmetics industry is dominated by formulations of synthetic origin, many of which are designed to enhance appearance but often come with undesirable dermatological side effects [01]. Among these, vanishing creams—typically oil-in-water (O/W) emulsions—are favored for their quick absorption and smooth, non-greasy texture. While they offer aesthetic benefits such as a brightening effect and smoother skin surface, prolonged use of synthetic formulations has been linked to skin irritation and hypersensitivity reactions [02]. In contrast, natural extracts have gained significant attention in the formulation of cosmetic products due to their biocompatibility, minimal side effects, and additional pharmacological properties. Herbal ingredients are not only safer for prolonged use but also provide therapeutic benefits such as antioxidant, antimicrobial, and anti-inflammatory effects [03]. Carrot (Daucus carota), a widely cultivated root vegetable, is rich in bioactive compounds such as beta-carotene, vitamin A, flavonoids, and phenolic acids. These constituents have been shown to support skin health, enhance wound healing, and delay signs of aging [04]. Daucus carota, particularly its ethanolic taproot extract, is reported to offer moisturizing and soothing effects when applied topically. The antioxidant properties of the root extract further contribute to skin rejuvenation by scavenging free radicals and protecting against environmental stressors [05]. Given these benefits, the present research aimed to design, develop, and evaluate a vanishing cream incorporating ethanolic taproot extract of Daucus carota. The goal was to formulate a product that provides hydration and skin protection without causing irritation, thus offering an alternative to synthetic formulations. Vanishing creams derive their name from their ability to disappear upon application, leaving behind a smooth, matte finish. As oil-in-water emulsions, they consist of fine droplets of oil dispersed in a continuous water phase, making them cosmetically elegant, easily washable, and less greasy compared to water-in-oil (W/O) emulsions [06]. The carrot-based vanishing cream formulated in this study was evaluated based on parameters such as consistency, spreadability, pH, and skin compatibility to determine its effectiveness as a safe and functional cosmetic product.
METHODOLOGY
Carrot (Daucus carota) is a rich source of essential nutrients including vitamin A, vitamin K1, vitamin B6, potassium, biotin, and bioactive compounds such as α-carotene and β-carotene, which have well-documented benefits for skin health. These constituents contribute to antioxidant protection, collagen preservation, acne prevention, and enhanced skin elasticity [07]. Specifically, vitamin A plays a critical role in cellular regeneration and protection against photoaging, while β-carotene supports the skin’s defense against UV radiation [08].
Plant Profile
Fig No.1 Daucus Carota
Materials Used
A. Excipients and Their Sources- All excipients including stearic acid, cetyl alcohol, triethanolamine, glycerine, methyl paraben, and propyl paraben were procured from reputed chemical suppliers such as SD Lab Chemical Centre (Mumbai) and Research Lab Fine Chem Industries (Islampur).
B. Equipment Used
Extraction Method
Fresh carrots were procured locally, washed, and chopped into small pieces. The extraction of bioactive compounds, primarily β-carotene, was carried out using the Soxhlet extraction technique. Approximately 100 ml of ethanol was used as the solvent for extraction due to its high polarity and compatibility with carotenoids [10]. The chopped carrot samples were ground and introduced into the Soxhlet apparatus for 30 minutes. Afterward, the mixture was filtered, and the filtrate was evaporated using a heating mantle to remove ethanol. The concentrated extract containing β-carotene was then collected and stored under refrigeration until formulation. Selection of the appropriate solvent is critical in carotenoid extraction, given their hydrophobic nature. Ethanol was chosen for this study due to its moderate polarity, effectiveness, and safety in cosmetic applications. Although solvents such as hexane, chloroform, and acetone have been used in other studies, ethanol remains a preferred option for herbal formulations to ensure consumer safety [11].
Fig. No: 02 Extraction Process
Formulation Procedure
Fig No 03 – Steps to create Vanishing Cream
Table No.01: Ingredients and their quantity taken
|
Sr.no |
Ingredients |
Quantity Taken (100 gm) |
|
1. |
Alc. Extract |
2 gm |
|
2. |
Glycerine |
6 ml |
|
3. |
Tri-ethanolamine |
1 ml |
|
4. |
Cetyl-alcohol |
0.5 gm |
|
5. |
Water |
78.3 ml |
|
6. |
Stearic acid |
12 gm |
|
7. |
Preservatives (Methyl paraben, propyl paraben) |
0.18 mg, 0.02 mg |
|
8. |
Perfume |
q.s |
RESULTS
The prepared vanishing cream formulation containing ethanolic extract of Daucus carota was evaluated for various physicochemical parameters including consistency, pH, spreadability, type of emulsion, extrudability, and skin compatibility. The goal was to determine whether the formulation met cosmetic standards for topical applications and exhibited no adverse effects on the skin.
1. Physical Appearance and Homogeneity
The formulation was smooth, free from grittiness, and showed good homogeneity. No phase separation or color change was observed during the storage period of 30 days at room temperature, indicating physical stability.
2. pH Determination
The pH of the formulation was found to be 6.3 ± 0.1, which is within the ideal pH range (5.5–7.0) for topical preparations. This pH value suggests compatibility with human skin and minimizes the risk of irritation or allergic response [17].
3. Spreadability Test
Spreadability determines the ease of application of cream on the skin. The average spreadability of the formulation was found to be 16.8 ± 0.4 g·cm/sec, indicating satisfactory ease of application and uniform distribution [18].
4. Viscosity
The viscosity was measured using a Brookfield viscometer and was observed to be 5,250 ± 250 cps (centipoise), which confirms the semi-solid nature and good consistency of the product. High viscosity aids in better retention and skin adherence [19].
5. Type of Emulsion
The dilution test confirmed that the formulation was an oil-in-water (O/W) emulsion, which is favorable for cosmetic use due to its non-greasy and washable nature [20].
6. Skin Irritation Test
No signs of erythema, edema, or irritation were observed in human volunteers up to 72 hours after application. This indicates that the cream is non-irritant and safe for use on human skin [21].
7. Extrudability Test
The formulation showed excellent extrudability from aluminum collapsible tubes under normal hand pressure, indicating appropriate semisolid consistency for user convenience.
Table No 02 - Evaluation of Vanishing Cream containing ethanolic extract.
|
Parameters |
Procedure |
Result |
|
Physical Properties- |
Colour Odour Appearance |
White Pleasant Smooth |
|
Washability - |
The cream was applied on the hand and observed under running water. |
Easily washable |
|
pH- |
Weighed 10 mg of cream, dissolved in 10 ml of d/wand its pH was measured with the help of digital pH meter. |
|
|
Viscosity- |
Viscosity of cream was determined by Brookfield viscometer |
27025cps |
|
Spreadability Test- |
The spread ability of formulated cream was judged by spread ability apparatus |
Uniform and easily spread.
|
|
Homogeneity- |
Homogeneity tested via visual appearance |
Homogenous |
|
Determination of Type of Emulsion-
i) Dye Test- |
The Amaranth solution was mixed with cream. Placed a drop of cream on a microscopic slide, covered it with a cover slip, and examined it under a microscope. If continuous phase appears in red colour the cream is o/w type. If dispersed phase appears red coloured globules the cream is w/o type. |
Continuous phase appears in red colour so the cream is o/w type.
|
|
ii) Formation of Creaming- |
Heated the sample vanishing cream in suitable test tube for 10 mins and observed the result. If creaming is upward then emulsion is o/w type. If the creaming is downward then the emulsion is w/o type. |
Creaming is upward hence o/w type emulsion.
|
|
Patch Test- |
About 1 gm of material to be tested was applied directly to the hands on skin. The cosmetic to be tested was applied on area of 1 sq.m of the skin. The site of patch was inspected after 24 hrs. As there was no reaction the test was repeated three times. If reaction is not observed on third application, the person may be taken as non- hypersensitive. |
No any reaction is observed.
|
|
Smear Type- |
The test was conducted after the application of cream on the skin. The smear formed can be oily or aqueous in nature. |
Non-Greasy in nature |
|
Determination of Emollience |
Emollience, slipperiness and amount of residue left after the application of fixed amount of cream was checked. |
No residue on skin |
CONCLUSION
In the present study, a vanishing cream formulation incorporating ethanolic extract of Daucus carota was successfully designed, developed, and evaluated using simple methods and minimal equipment. The formulation demonstrated promising stability and cosmetic acceptability. Key evaluation parameters such as pH (6.70 and 6.98), spreadability (11.30 g·cm/sec and 13.33 g·cm/sec), and tube extrudability confirmed the suitability of the product for topical application. Notably, the carrot-based cream maintained good physical integrity, while the standard formulation showed slight hardening at 50°C [22]. No signs of skin irritation, erythema, or adverse reactions were observed following 72 hours of application, indicating excellent dermatological safety of the formulation [23]. These results affirm the non-irritant, non-greasy, and washable nature of the carrot-based cream. The active herbal component, Daucus carota, was selected for its recognized cosmetic properties including anti-aging, anti-wrinkle, moisturizing, and skin-rejuvenating effects. This is attributed to its high content of bioactive nutrients such as beta-carotene, alpha-carotene, and vitamins A, K, B6, and C [24]. Phytochemical screening of the ethanolic extract revealed the presence of several beneficial constituents’ alkaloids, carbohydrates, phenolic compounds, flavonoids, and proteins which contribute to the observed antioxidant and anti-inflammatory effects [25–27]. The formulation was prepared using the melt emulsification technique and passed all standard evaluation tests. The cream displayed oil-in-water (O/W) emulsion behavior, which is preferable for cosmetic use due to its non-greasy texture and ease of removal [28]. Table 1 results confirmed that the cream had a pleasant odour, smooth appearance, appropriate viscosity, and pH compatible with skin, making it highly acceptable for regular use. Overall, the prepared herbal vanishing cream showed enhanced functional and cosmetic properties. It effectively moisturizes the skin, supports anti-aging, and provides a protective, soothing barrier. Additionally, it complies with key quality parameters such as Spreadability, pH, viscosity, and stability. The cream was found to be non-irritant and well tolerated by the skin, confirming its safety profile. This study demonstrates that a carrot-based vanishing cream can be a valuable natural alternative to synthetic formulations. It combines nutritional and cosmetic benefits, making it a multifunctional skincare product. While initial findings are promising, further clinical trials and long-term stability studies are recommended to validate efficacy, safety, and consumer acceptability on a larger scale [29].
REFERENCES
Aishwarya Kholkumbe*, Monika Ghodke, Ekta Waghmare, Adesh Tandale, Vrushabh Patil, A Novel Approach to Herbal Skincare: Preparation and Evaluation of Daucus carota-Based Vanishing Cream, Int. J. of Pharm. Sci., 2025, Vol 3, Issue 7, 1940-1948. https://doi.org/10.5281/zenodo.15901415
10.5281/zenodo.15901415