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Abstract

Aging skin is caused by a persistent process of degradation and harm to DNA and proteins in cells. Our study's primary goal is to create a cream with polyherbs to prevent aging. In our preparation, we used herbal ingredients such as tulsi leaves, butterfly pea flowers, aloe vera gel, oil of turmeric. The presence of anthocyanins is indicated by the fact that butterflies contain cyanin, yanin, and anthocyanins, which are natural antioxidants that slow down the Tulsi leaves are used to treat skin aging and as an antibacterial. VestaVeuse moisturiturmeric, zer, and oilluminizing.sing. minimize acne and lighten dark circles. These are a few of the basic medications used in the cream's formulation. In assessing the quality of formulation [f1–f5] was carried out using various parameters, such as pH, viscosity,phase separationon and spreadability. The formulation of n.F3 is superior to all others formulation. The anti-aging cream’s physical characteristics remained unchanged. The formulation had no phase and good spreadability. separation and high uniformity. It is discovered that the cream’s viscosity is sufficient. The formulation of the polyherbal cosmetics is safe, stable at temperature, and can be applied to the skin without risk. Skin aging is brought on by a persistent degradation process brought on by damage to cellular DNA and protein Aging is classified into two distinct categories: “sequential skin aging” and “photo-aging.” Both types have distinct clinical and historical traits. Butterfly pea flowers are rich in antioxidant flavonoids and phenolic compounds that protect the skin from free radical-induced oxidative damage. Maximizing allocation was the aim of this study. mixture that contains butterfly pea flower extract (BPFE), triethanolamine (TEA), and as emulsifiers and to evaluate stearic acid's antioxidant potential. Eight different lotion formulas were developed using the Simplex Lattice Design method in Design Expert v13.0, with a focus on optimizing the proportion of TEA to stearic acid. Antioxidant activity is measured using DPPH (2,2-diphenyl-1- picrylhydrazyl) at a wavelength of 516 nm. Crucial elements such as pH, adhesion, and spreadability and measurements of viscosity were taken. Maceration was the method used to extract BPFE.Response efficiency. The refined mixture showed 6.26±0.38 cm of spreadability, adhesion of 3.2±0.2 s, 120 dPas of viscosity, and 7.19±0.005 pH. Both the optimized lotion and BPFE Strong antioxidant activity was demonstrated by the formulation, with IC50 values of 59.87±0.802 µg/ml and 84.52±1.418 µg/ml, in that order. The improved BPFE lotion formula shows encouraging possibility of becoming a coproduct. Growing awareness of artificial sunscreen’s harmful effects Agents are paying more attention to herbal alternatives. This study ?plans to develop and evaluate a herbal cream using Clitoria. Þ?8Ternatea, or blue pea flower extract, is well known for its potent antioxidant and photoprotective properties. The extract was incorporated into a cream-based formulation using natural excipients. Phytochemical cream confirmed itsè³ presence of flavonoids, anthocyanins, and phenolic compounds. Compounds that enhance the plant’s ability to absorb ultraviolet light. Concerning anti-aging cream. Thus polyherbal cosmetics formulation is safe to use was stable at room temperature and can be safely used on the skin.

Keywords

Butterfly pea flower, Herbal cosmetics, Aloe Vera, Anti-Aging cream, Skin care, Clitoria ternatea, Antioxidant, Natural Formulation, Photoprotective, Phytochemical

Introduction

Taking care of their skin has always been important to humans. Youth is indicated by smooth, supple skin. However, collagen, a structural protein, is responsible for this flawless appearance. Collagen acts as a helpful cushion to keep the skin supple and forms an even layer underneath it. The thinning of the skin and the unevenness of the collagen padding beneath cause wrinkles to appear as we age. Premature aging can be brought on by excessive sun exposure, tobacco smoke, and dehydration. It is an inevitable part of aging naturally. The aging process can be somewhat slowed down with the right skin care and protective measures, degree. One of the primary causes of the collagen layer's degradation is oxidative stress. As a result of this need, skin care products have been continuously improved upon and created. preparations for cosmetic purposes. Preparations for skin care have existed since ancient times. It has long been practiced to preserve and beautify the skin by using slaves and unguents. They were mostly made by breaking down gums, roots, flowers, and fragrant resins with fats and oils. Galen, a Greek physician, made the first significant modification in the second century. By adding water to his sales, A.D. That readiness was regarded as the cornerstone. contemporary cold and cleansing creams. However, throughout the ages, a constant Changes were made, but the fundamental idea stayed the same. It's now so It's crucial that significant resources and time are consistently allocated to research pertaining to with how different substances and treatments affect the skin and to create better skin care getting ready.  Since wide-spectrum additives such as emulsifying agents are now readily available, and with the advancement of numerous methods, cream preparation has become incredibly straightforward. The majority of creams are emulsions, and they can range in consistency from liquid to spreadable solid. Every skin care product can be categorized using one of the following methods:

1. Depending on its purpose, such as cleansing, foundation, massage, etc.

2. Based on distinctive qualities, such as vanishing creams, cold creams, etc.

3. Based on the type or nature of emulsion.

Function-based classification is the most commonly used.

The cream can be categorized in the following ways based on its function:

1.Cold and cleansing creams.

2.Vanishing creams and foundation

3. Massage and night creams.

4. Creams for the hands and body.

5. General and all-purpose creams.

1. Cold and cleansing creams: A cleansing lotion or cream is necessary to remove makeup, dirt on the face, water and oil, and soil that dissolves in oil effectively, mostly from the throat and face. An effective cleansing cream with the right formula should be able to eliminate Cosmetics such as face powder; rouge, foundation bases, cake makeup, lipstick, and makeup were applied quickly and effectively. Cleaning cream overcomes the drawbacks of conventional soap, such as that it makes the skin appear dry, while the soap makes the skin appear moist. The purification the following mechanism in the cream allows it to easily remove the chemicals of the facial makeup by removing or dissolving the oily binding substances that dirt or pigments on the skin.

Cleansing cream types

1. White, beeswax-borax-based emulsified cold creams

2. Translucent, anhydrous, liquefying type, and composed of a blend of hydrocarbon-based waxes and oils.

2. Foundation and creams that disappear

a. Disappearing creams and foundation: These products are well-liked for several goals. The names that have been derived have been informed by the functions. Dissolving creams are vanishing creams because they seemed to disappear after being applied to the skin. The preparations are based on stearic acid, which is partially specified with an alkali and A considerable amount of water is used to emulsify the soap and the leftover stearic acid. Observing application The cream leaves a tacky, dry residue on the skin and dries it out. the skin, which is why stearic acid soap-based creams are still recommended. Along with greasy skin conditions.

b. Creams for foundation: These products make the skin look smooth. Before applying face powder and other cosmetics, use an emollient base or foundation. preparations; they have a strong holding power and aid in the powder’s skin adherence. power, they should be greasy, have an occlusive effect, and apply to the face like a film. The preparation’s humectant aids in the powder’s preservation.

3. Night creams and massages: It’s critical to nourish the skin to maintain the either as a remedy for dry skin or as a natural characteristic of the skin. To enhance foods that are good for the skin and to take care of your skin. They employ various creams made of various ingredients. The typical one characteristic unites all creams: they are typically applied to the skin and left for a number of hours, day, and night all play a part in repairing the damaged surface. Harmed by exposure to exposure solutions, soap, or other chemicals. Creams for the night they are typically applied at night for feeding and skin preservation.

There are two ways that emollient action can be accomplished.

1.Preventing the skin from losing water, which increases the water content inside.

2.Increasing the skin’s water content by drawing in water from the atmosphere by using humectant substance. The process that draws water from the atmosphere is The advantage of maintaining the skin’s water content is that it calms and moisturizes the skin of massage creams and night.

4. Body and head creams: Water exposure can affect the skin of the hands and other body parts. Soap and detergents are used to remove lipids and other secretions from the skin. Dry, scaly skin: Infections brought on by microbes can cause dermatitis, so in order to control all body and hand creams, . are employed

The following are the hands’ and body’s main functions:

1.Coat the skin with oil to protect it.

2.Keep your skin tone smooth but not oily.

3.It’s easy to apply.

5. All Purpose Creams: In recent times, there has been a notable growth in the use of products commonly known as all-purpose creams. These are also referred to as sports creams, as they were originally used by athletes for outdoor and physical activities. They possess a slightly oily yet non-greasy consistency, allowing them to spread smoothly on the skin and form a thin protective film. All-purpose creams serve multiple roles — they can act as nourishing or night creams, protective creams to prevent or soothe sunburn, or remedies for rough and dry skin areas. When applied lightly, they also function effectively as hand creams or foundation creams. Due to their versatile nature, they are termed all-purpose creams.

Their formulation is specifically designed to:

  1. Function as a foundation cream, providing a suitable base for makeup.
  2. Act as a hand cream , offering emollient and moisturizing properties.
  3. It acts as a protective cream that creates a uniform, breathable layer over the skin.
  4. It is also intended to improve and smoothen rough skin texture.

The purpose of the current study was to prepare and assess an antiaging cream containing extracts of blue pea flower , Alovera gel , conflower  powder,  distilled water.

Excipients And Herbal Ingredients With Their Role:

Table no.1 Role of Ingredients

Sr. No

Ingredients

Role

1.

Butterfly pea flower extract

Antioxidants, Anti-inflammatory

2.

Aloe Vera gel

Anti-ageing, anti-inflammatory, moisturizer, reduce acne and pimples.

3.

Cornflower powder

calm skin irritation, reduce redness, and refresh tired or sensitive skin.

4.

Moraccan Argan Oil

Natural emollient, moisturizer, and antioxidant

5.

Vitamin E Capsule

Skin-conditioning, anti-aging, and protective agent

METHOD:

1.Extraction process:

Prepare the flowers:

Use fresh or dried butterfly pea flowers. If using fresh flowers, Rince them to improve dirt and impurities.

2.  Maceration (Soaking)

Add the flowers to a glass or stainless-steel container. Pour distilled water over the flowers in 10 to 20 ml of water per gram of flowers. Stir the mixture gently to ensure even contact.

3.Extraction Time and Temperature:

Let the mixture sit at room temperature (25 -30°c) for 6-24 hours. For better efficiency, gentle stirring every few hours helps     release the anthocyanins. Higher temperature (40-50°c) can improve extraction efficiency but may degrade anthocyanins over time.

4.Filtration:

After maceration, filter the extract using cheesecloth, a fine sieve, or filter paper to remove the solid residues

5.Concentration:

If more concentrated extract is needed, evaporate some of the water using a low-temperature method like vaccum evaporation or freeze -drying.

6.Storage:

Store the extract in an airtight dark-colored container to protect it from light degradation. 

Refrigerate(4°c) to extend shelf life

Fig no.1 Extraction of Butterfly Pea Flower

Formulation Table:

Table no.2 Formulation

Sr. No

Ingredient

%w/w

Weight (g) per 100 g batch

1.

Aloe Vera gel (standardized, 100%)

60.0

60.0

2.

Butterfly pea flower extract (aqueous extract)

2.0

2.0

3.

Moroccan Argan oil (cold-pressed)

8.0

8.0

4.

Cornflower powder (finely milled petals)

1.0

1.0

5.

Vitamin E (tocopherol, from capsules)

0.5

0.5

6.

Distilled water (q.s. to 100)

17.8

17.8

7.

Preservative (phenoxyethanol + ethylhexylglycerin)

0.5

0.5

8.

Fragrance / Essential oil (optional)

0.2

0.

Flower Extract

The growing demand for natural skincare has inspired the formulation of herbal night creams using plant-based ingredients that nourish and protect the skin. Among them, blue pea flower extract (Clitoria ternatea), or butterfly pea flower, is valued for its strong antioxidant, anti-inflammatory, and anti-aging properties. Rich in anthocyanins, flavonoids, and polyphenols, it helps repair skin, protect against environmental damage, and maintain a youthful glow.

When added to an anti-aging night cream, it enhances skin renewal, smoothness, and elasticity, reducing fine lines and improving texture. Free from parabens and artificial fragrances, the cream is safe for all skin types, including sensitive skin.

Quality testing includes stability, pH, spreadability, and while evaluations of viscosity, Greasiness and radiance confirm its effectiveness.

General Method for Preparation of Herbal Moisturizer

Material and method(O/W,W/O)

Add the required quantity of butterfly pea flowers in sufficient amount of base water and prepare a solution by heating on water bath

In the above solution,add required quantity of Herbal extract

Add Aloe Vera gel into solution 1, and also add cornflower powder when both the phases get mixed properly, add moroccan Argan Oil  as natural emollient In this above mixture add Vitamin E Capsule as a protective agent

The formulated anti-aging cream kept aside for about an hour in cool and dry place indirect to sunlight till it sets completely and was used after 48 hours after keeping at room temperature for stability and analytical testing

Packed in container and store in cool place

Application:

? Skin softening

? Smoothing

? Moisturizing

? Anti-allergic

? Antiseptic

? Astringent

? Cleaning

? Protective

? Scabicidal

? Local anaesthetic

? Parasiticide

? Germicide

? Antifungal

? Fairness

? Antiaging

Fig no.2 Butterfly Pea Flower Cream

Evaluation Of Cream

1.Appearance:

The appearance of the butterfly pea flower cream was examined after application, noting characteristic such as color, texture, odor, state.

2.Irritancy:

Mark the area (1 cm2) on the left-hand dorsal surface. Then the cream was applied to that area and the time was noted. Then it is checked for irritancy, erythema, and edema if any for an interval up to 24 h and reported

3.Wash ability:

A small amount of cream was applied on the hand and it is then washed with tap water

4.PH:

 A small amount of the cream was applied to PH paper and compared against a standard pH color chart to estimate the pH level.

5.Viscosity:

Viscosity of cream was done by using Brooke field viscometer at a temperature of 25? using spindle No. 63 at 2.5RPM

6.Spread ability:

The Spreadability was expressed in terms of time in seconds taken by two slides to slip off from the cream, placed in between the slides, under certain load. Lesser the time taken for separation of the two slides better the spreadability. Two sets of glass slides of standard dimension were taken. Then one slide of suitable dimension was taken and the cream formulation was placed on that slide. Then other slide was placed on the top of the formulation. Then a weight or certain load was placed on the upper slide so that the cream between the two slides was pressed uniformly to form a thin layer. Then the weight was removed and excess of formulation adhering to the slides was scrapped off. The upper slide was allowed to slip off freely by the force of weight tied to it. The time taken by the upper slide to slip off was noted.

Spread ability = m × l/t 

Where, 

M= Standard weight which is tied to or placed over the upper slide (30g) 

L= length of a glass slide (5cm) 

T= time taken in seconds. 

7.Greasiness:

Here the cream was applied on the skin surface in the form of smear and checked if the smear was oily or grease-like

8.Dye test:

Dye test confirmed that all the cream formulations were o/w emulsions

9.Homogeneity:

All formulations produce uniform distribution of extracts in cream. This  was confirmed by visual appearance and by touch

CONCLUSION:

In conclusion, butterfly pea flower cream is a skincare product that harnesses the benefits of butterfly pea flower extract, known for its antioxidant anti-aging, and soothing properties, when infused into a cream, it helps hydrates, rejuvenate, and protect the skin. The cream is often rich in natural ingredients like plant-based oils and aloe Vera gel, which contribute to its moisturizing and smoothing effects. Regular use of butterfly pea flower cream may promote a healthier, more radiant complexation while offering potential anti-inflammatory benefits. Furthermore, combining the extracts of Curcuma longa, Solanum lycopersicum, and Carica papaya in various proportions provides a multifunctional skincare effect, offering benefits such as skin whitening, anti-wrinkle, anti-aging, and sun-protective actions. While a single plant extract may have limited efficacy, the synergistic blending of multiple herbal extracts enhances their overall therapeutic and cosmetic potential. Thus, the formulated herbal cream demonstrates improved effectiveness and broader skincare benefits compared to individual plant extracts.

REFERENCES

  1. Ashwini, S. D., Somishwar, S. K. and Shweta, S. S. Formulation and evaluation of vanishing herbal cream of crude drugs. American J. Ethnomedicine, 2014; 1(5): 313-318.
  2. Das, K., Dang, R. and Manjunath, U. M. Formulation and evaluation of a novel herbal gel of stevia extract. Iranian J. of Dermatol, 2009; 12(4): 117-122.
  3. Gautam, S., et al., Fomulation and evaluation of herbal hair oil. Int. J. Chem. Sci., 2012;1(1): 261-267.
  4. Johansson, S. G., Hourihane, JO’B., Bousquet, J., Bruijnzeel-Koomen, C., Dreborg, S.and Haahtela, T. A revised nomenclature for allergy. An EAACI position statement from the EAACI nomenclature task force, 2001; 56: 813-824.
  5. Kaur Loveleen Preet and Tarun Kumar Guleri. Topical Gel: A recent approach for novel drug delivery. Asian Journal of Biomedical and Pharmaceutical Sciences, 2013; 3(17): +1-5.
  6. Ganceviciene, R., Liakou, A. I., Theodoridis, A., Makrantonaki, E., & Zouboulis, C. C. “Skin Anti-Aging Strategies.” Dermato-Endocrinology, 2012; 4(3): 308-319.
  7. Clitoria ternatea Flower and Its Bioactive Compounds: Potential Use as Microencapsulated Ingredient for Functional Foodsby Ribi Ramadanti Multisona, Shwetali Shirodkar.
  8. Mukherjee, P. K., Maity, N., Nema, N. K., & Sarkar, B. K. “Bioactive Compounds from Natural Resources against Skin Aging.” Phytomedicine, 2011.
  9. Natural anti-aging skincare: role and potential by Idris Adewale Ahmed, Maryam Abimbola Mikail, Norhisam Zamakshshari.
  10. Shah RN, Methal BM, A Hand book of Cosmetics.
  11. Gupta, Girish & Chahal, Jagbir &  Bhatia, Manisha.2010.
  12. Lachman L, Lieberman H, Kanig J. The Theory and Practice if Industrial Pharmacy.1991.
  13. Loden M. Role of skin care in maintaining barrier function. Dermatol Ther. 2003.
  14. Surya Prabha, Matangi, Santhosh Aruna. Mamidi, Gulshan Raghavamma Rama rao Nadendla.2Ther.
  15. Akash S. Mali, Karekar P, Yadav A.V.2015.
  16. Dr K. W. Quirin, Herbal Extracts in Support of Natural Cosmetics Preservation. Cosmetic
  17. Science Technology. Flavex Naturextrakte GmbH, Germany, 2007.
  18. Sahu T, Patel T, Sahu S, Gidwani B,“Skin Cream as Topical Drug Delivery System: Review” Journal of Pharmaceutical and Biological Sciences, Published by Atom and Cell Publishers, ISSN: 2320-1924
  19. James WD, Berger TG, Elston DM. ndrews? diseases of the skin: Clinical dermatology.
  20. Philadelphia: Elsevier Saunders, 2006; 10.
  21. R.N. Shah, B.M. Methal, 2006 A Handbook of Cosmetic, Vallabh Prakashan.
  22. Nikhil Nitin Navindgikar, K.A. Kamalapurkar, Prashant S. Chavan. 2020.
  23. Ashwini S. Dhase, Somishwar S. Khadbadi and Shweta S. Saboo. Kirtikar KR and Basu BD 1980.
  24. https://share.google/images/IWxrV9rOvz2cOZeAY
  25. Boyd AS, Naylor M, Cameron GS, The effects of chronic sunscreen use on the histologic changes of dermatoheliosis. J Am Acad Dermatol., Dec 1995; 33(6): 941-946.
  26. DeBuys HV, Levy SB, Murray JC, Modern approaches to photo protection. Dermatol Clin., Oct 2000; 18(4): 577-590.
  27. Diffey BL and Grice J. The influence of sunscreen type on photo protection. Br J Dermatol., Jul 1997; 137(1): 103-105.
  28. Dromgoole SH and Maibach HI. Sunscreening agent intolerance: contact and photo contact sensitization and contact urticaria. J Am Acad Dermatol., Jun 1990; 22(6): 1068-1078.
  29. Fotiades J, Soter NA and Lim HW. Results of evaluation of 203 patients for photosensitivity in a 7.3-year period. J Am Acad Dermatol. Oct 1995; 33(4): 597-602.
  30. Mithal BM and Saha RNA. Hand book of cosmetics, first edition, reprint, 2007; Vallabh Prakashan, Delhi, 122-124.
  31. K. Suria prabha et al., comparative invitro relaease of diclofenac sodium gel international journal of life science & pharma research, Jul-Sept, 2012; 2(3).
  32. Tikshdeep Chauhan et al., Design and Evaluation of Diclofenac Sodium Gel international journal of pharmaceutical and chemical sciences, jan-mar, 2013; 2(1).
  33. Dheeraj T Baviskaret al., In Vitro and In Vivo Evaluation of Diclofenac Sodium Gel Prepared with Cellulose Ether and Carbopol 934P Tropical Journal of Pharmaceutical Research August, 2013; 12(4): 489-494.
  34. Sujith S Nair1 et al., Formulation and Evaluation of Herbal Creamcontaining curcuma longa international journal of pharmaceutical and chemical sciences, Oct-Dec, 2012; 1(4):
  35. Pallab Dasgupta and Amartya De et al., Comparative Standardization Study of Two marketed Ashwagandha Churna Formulation International Journal of Research in Pharmaceutical and Biomedical Sciences, Apr–Jun, 2012; 3(2).
  36. Arun Rasheed et al., Standardisation of herbal formulation International Journal of. Phytotherapy, 2012; 2(2): 74-88.
  37. Agatha Betsyet al., Effect of Ashwagandha (Withania somnifera) Root Powder Supplementation in Treatment of Hypertension Ethno Med, 14, 2012; 6(2): 111-115.

Reference

  1. Ashwini, S. D., Somishwar, S. K. and Shweta, S. S. Formulation and evaluation of vanishing herbal cream of crude drugs. American J. Ethnomedicine, 2014; 1(5): 313-318.
  2. Das, K., Dang, R. and Manjunath, U. M. Formulation and evaluation of a novel herbal gel of stevia extract. Iranian J. of Dermatol, 2009; 12(4): 117-122.
  3. Gautam, S., et al., Fomulation and evaluation of herbal hair oil. Int. J. Chem. Sci., 2012;1(1): 261-267.
  4. Johansson, S. G., Hourihane, JO’B., Bousquet, J., Bruijnzeel-Koomen, C., Dreborg, S.and Haahtela, T. A revised nomenclature for allergy. An EAACI position statement from the EAACI nomenclature task force, 2001; 56: 813-824.
  5. Kaur Loveleen Preet and Tarun Kumar Guleri. Topical Gel: A recent approach for novel drug delivery. Asian Journal of Biomedical and Pharmaceutical Sciences, 2013; 3(17): +1-5.
  6. Ganceviciene, R., Liakou, A. I., Theodoridis, A., Makrantonaki, E., & Zouboulis, C. C. “Skin Anti-Aging Strategies.” Dermato-Endocrinology, 2012; 4(3): 308-319.
  7. Clitoria ternatea Flower and Its Bioactive Compounds: Potential Use as Microencapsulated Ingredient for Functional Foodsby Ribi Ramadanti Multisona, Shwetali Shirodkar.
  8. Mukherjee, P. K., Maity, N., Nema, N. K., & Sarkar, B. K. “Bioactive Compounds from Natural Resources against Skin Aging.” Phytomedicine, 2011.
  9. Natural anti-aging skincare: role and potential by Idris Adewale Ahmed, Maryam Abimbola Mikail, Norhisam Zamakshshari.
  10. Shah RN, Methal BM, A Hand book of Cosmetics.
  11. Gupta, Girish & Chahal, Jagbir &  Bhatia, Manisha.2010.
  12. Lachman L, Lieberman H, Kanig J. The Theory and Practice if Industrial Pharmacy.1991.
  13. Loden M. Role of skin care in maintaining barrier function. Dermatol Ther. 2003.
  14. Surya Prabha, Matangi, Santhosh Aruna. Mamidi, Gulshan Raghavamma Rama rao Nadendla.2Ther.
  15. Akash S. Mali, Karekar P, Yadav A.V.2015.
  16. Dr K. W. Quirin, Herbal Extracts in Support of Natural Cosmetics Preservation. Cosmetic
  17. Science Technology. Flavex Naturextrakte GmbH, Germany, 2007.
  18. Sahu T, Patel T, Sahu S, Gidwani B,“Skin Cream as Topical Drug Delivery System: Review” Journal of Pharmaceutical and Biological Sciences, Published by Atom and Cell Publishers, ISSN: 2320-1924
  19. James WD, Berger TG, Elston DM. ndrews? diseases of the skin: Clinical dermatology.
  20. Philadelphia: Elsevier Saunders, 2006; 10.
  21. R.N. Shah, B.M. Methal, 2006 A Handbook of Cosmetic, Vallabh Prakashan.
  22. Nikhil Nitin Navindgikar, K.A. Kamalapurkar, Prashant S. Chavan. 2020.
  23. Ashwini S. Dhase, Somishwar S. Khadbadi and Shweta S. Saboo. Kirtikar KR and Basu BD 1980.
  24. https://share.google/images/IWxrV9rOvz2cOZeAY
  25. Boyd AS, Naylor M, Cameron GS, The effects of chronic sunscreen use on the histologic changes of dermatoheliosis. J Am Acad Dermatol., Dec 1995; 33(6): 941-946.
  26. DeBuys HV, Levy SB, Murray JC, Modern approaches to photo protection. Dermatol Clin., Oct 2000; 18(4): 577-590.
  27. Diffey BL and Grice J. The influence of sunscreen type on photo protection. Br J Dermatol., Jul 1997; 137(1): 103-105.
  28. Dromgoole SH and Maibach HI. Sunscreening agent intolerance: contact and photo contact sensitization and contact urticaria. J Am Acad Dermatol., Jun 1990; 22(6): 1068-1078.
  29. Fotiades J, Soter NA and Lim HW. Results of evaluation of 203 patients for photosensitivity in a 7.3-year period. J Am Acad Dermatol. Oct 1995; 33(4): 597-602.
  30. Mithal BM and Saha RNA. Hand book of cosmetics, first edition, reprint, 2007; Vallabh Prakashan, Delhi, 122-124.
  31. K. Suria prabha et al., comparative invitro relaease of diclofenac sodium gel international journal of life science & pharma research, Jul-Sept, 2012; 2(3).
  32. Tikshdeep Chauhan et al., Design and Evaluation of Diclofenac Sodium Gel international journal of pharmaceutical and chemical sciences, jan-mar, 2013; 2(1).
  33. Dheeraj T Baviskaret al., In Vitro and In Vivo Evaluation of Diclofenac Sodium Gel Prepared with Cellulose Ether and Carbopol 934P Tropical Journal of Pharmaceutical Research August, 2013; 12(4): 489-494.
  34. Sujith S Nair1 et al., Formulation and Evaluation of Herbal Creamcontaining curcuma longa international journal of pharmaceutical and chemical sciences, Oct-Dec, 2012; 1(4):
  35. Pallab Dasgupta and Amartya De et al., Comparative Standardization Study of Two marketed Ashwagandha Churna Formulation International Journal of Research in Pharmaceutical and Biomedical Sciences, Apr–Jun, 2012; 3(2).
  36. Arun Rasheed et al., Standardisation of herbal formulation International Journal of. Phytotherapy, 2012; 2(2): 74-88.
  37. Agatha Betsyet al., Effect of Ashwagandha (Withania somnifera) Root Powder Supplementation in Treatment of Hypertension Ethno Med, 14, 2012; 6(2): 111-115.

Photo
Renuka Damodhar
Corresponding author

Shraddha Institute of Pharmacy, Washim.

Photo
Swati Kanoje
Co-author

Shraddha Institute of Pharmacy, Washim.

Photo
Dr. Swati Deshmukh
Co-author

Shraddha Institute of Pharmacy, Washim.

Renuka Damodhar*, Swati Kanoje, Dr. Swati Deshmukh, Formulation and Evaluation of Herbal Anti-Aging Cream Utilizing Clitoria Ternatea, Int. J. of Pharm. Sci., 2025, Vol 3, Issue 11, 425-433 https://doi.org/10.5281/zenodo.17519863

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