Antifungal Emulgel: A Novel Approach for Topical Treatment of Fungal Infections

Abstract

The development of Medicle Administration systems approached Dermatological conditions, that provides target and effective administration directly in the affected areas of the skin. Emulgel Formulations, by combining the properties of emulses and graits, have a promising solution in the field. This prograve study on the formulation of an emulgel containing fiorda, an antifungal agent, also purposefully improve their therapeutic against the infects of the sheet The form of formulity designs as xanthan gum for their optimization of the integration of emulsion to ensure stability and efficiency. Various evaluation settings, including physical appearance, pH, distribution of the drug kinetics, they were meticulously examined to characterize the emulgel performance. The solubility studies confirmed the flower ability in Glycol Dietylen Monomet Etero (Transcutol P), optimizing their own properties for the current application. UV Spectrometer has tried the identity and the guishness of drug, essential to the accuracy of the wording. Emulgel presented desired features as the administrated penetration, controlled release and improve the respect of traditional formations. Generally, this study inhabited the pulfillment of empolitity as opportunity prepait of the current system and efficient, the applaimal system.

Keywords

Introduction

A medicine administration applied by the actual way is used to medicines directly in the overall skin circulation. To handle skin diseases, therapeutic substances are applied instead of the skin. A growing number of people choose the distribution of the skin drugs because of their affort and comfort. It is possible administering medicines in place and systematically across the skin, which serves as a wisely mechanical obstacle to the penetration of the drug. People had to face some diseases that have affected their health and well-what is the antiquity. The scientific search and the invention of certain treatments, the taxials and shipping systems are motivated by the search to heal these disorders. When you take a drug needed to treat a disease, there are many ways to administer in order to achieve a therapeutic response. The nature and extent of the disease have an impact on the administration technique. Current treatment is generally preferable to skin problems. A current application technique provides a shape of a wide medicine varieties directly on the skin to achieve the action of medicine. The faster and easiest method of drug administration with the multi body, including the skin, the vagina, Uptalmology and Rept. They can be used as a wide range of cosmetic and dermatological treatments on the healthy skin.Antifungal emulgel is a type of topical gel specifically formulated to treat fungal infections on the skin. It helps to relieve symptoms such as itching, redness and irritation.

Luliconazole stops the synthesis of ergosterol by inhibiting the enzyme lanosterol demethylase is the intermediate required for the ergosterol. Ergosterol is the major component of the fungus cell membran

Luliconazole

Objective:

1. To formulate a stable and effective Emulgel for topical delivery.
2. To select suitable excipients for optimal consistency, stability, and drug release.
3. To enhance skin penetration and retention of the active ingredient.
4. To evaluate the Emulgel’s physicochemical properties, drug content, and in vitro drug release.
5. To assess stability and skin compatibility of the final formulation.

MATERIAL AND METHOD:

Materials:

Methods:

1. Drug Phase: Luliconazole was dissolved under stirring in Propylene glycol till it forms a clear solution.
2. Oil Phase: BHT was dissolved in isopropyl myristate under slow stirring. Mineral oil and Tween 80 were added under slowing stirring and mixed properly.
3. Drug phase was added in oil phase under stirring and mixed for 10min.
4. Aqueous Phase: Xanthan gum was dispersed under stirring in the mixture of Tween 20 and purified water.
5. Emulsification: Aqueous phase was added to Oil phase under homogenization and homogenization continued for 30min at homogenization speed 3000-4000 RPM.
6. Addition of Sodium Benzoate: Sodium Benzoate was dissolved in Purified water. The resulting solution was added to emulsified bulk under homogenization and homogenization continued for 30min at homogenization speed 3000-4000 RPM.
7. Mixing: Final bulk was mixed under stirring for 30min.

Fig. Formulated Emulgel Preformulation Study

Evaluation of Emulgel:   

Preformulation Study

Organoleptic properties- The pure drug sample was studied for their organoleptic properties like colour, odour, taste and crystallinity and pH.

Determination of Melting Point- Melting point of drug sample was determined by using melting point apparatus. Drug sample was filled in one end open capillary tube. The capillary was placed in melting point apparatus and gradually temperature rises when drug sample was melted the melting point of sample powder was recorded.

Determination of λmax By UV spectrophotometer- 100mg of Luliconazole sample was

weighed and transferred to 100ml volumetric flask and adding 1:1 methanol: water as solvent upto the mark to give 1000μg/ml solution. 10ml of the above solution was pipetted out in a 100ml volumetric flask and diluted up to the mark. From this 1ml of the solution was pipetted out and transferred into a 10ml volumetric flask and diluted up to the mark with methanol to form 10μg/ml that was scanned in the range of 200-400nm using UV-visible Double Beam Spectrophotometer (Shimadzu 1800).

Preparation of Calibration curve of Luliconazole- The calibration curve of luliconazole were prepared in distilled water and methanol by using Shimadzu 1800 UV visible spectrophotometer. Accurately weighed 100mg of luliconazole was transferred into a 100ml volumetric flask and adding 1:1 methanol: water as solvent upto the mark to give 1000µg/ml standard stock solution of luliconazole. 10ml of standard stock solution was transferred to a 100ml of volumetric flask and volume was made up to the mark to give 100µg/ml of working solution from which further dilutions of 2-20µg/ml was prepared by pipetting out 0.2, 0.4, 0.6, 0.8, 1, 1.2, 1.4, 1.6, 1.8 and 2 ml of working solution to different 10ml volumetric flasks.

Observed absorption maxima, λmax 299nm was used for further analysis of absorption for concentration ranging from 2 to 20µg/ml. The linear plot was constructed and correlation coefficient value was determined.

Partition Coefficient- The partition coefficient determination of luliconazole was performed

using n-octanol as the oil phase and water (1:1) as the aqueous phase. The two phases were mixed in equal quantities (50 ml) by adding 50 mg of drug in a separating funnel and was saturated with each other at room temperature for 24 hour to separate the two phases. The test compound in each phase was sampled and quantitated using UV spectroscopy. The ratio of obtained concentration in octanol phase to the concentration in the buffer phase was determined

RESULT AND DISCUSSION:

Solubility of drug

Luliconazole Soluble in ethanol, Diethylene glycol monoethyl ether, dimethyl sulfoxide and chloroform and insoluble in water.

Melting point

The melting point of Luliconazole was found to be 150.5-151.5°C which complied with the standard, thus indicating the purity of obtained drug sample.

Fig. Uv spectrum of Luliconazole

Solution of 10µg/ml in methanol showed a peak at 296 nm with absorbance 0.5650.

The absorbance maximum at 296 nm is characteristics of the Luliconazole molecule and this confirms the identification of Luliconazole molecule.

Construction of calibration curve by UV Spectrophotometer In Methanol

Absorbance values of standard solutions of Luliconazole in beer’s range (0-25μg/ml) in methanol. The curve was found to be linear in the Beer’s range between 0 – 25μg/ml at 294 nm. The correlation coefficient (R2) obtained was 0.998 and equation was y = 0.038x + 0.036.

Table. Concentration of Luliconazole vs Absorbance in methanol

Fig. Calibration curve of Luliconazole in methanol

Evaluation:

CONCLUSION

In this study, an attempt was made to formulate Emulgel formulation of Econazole for Topical delivery.

The physical stability study of Luliconazole and combination of Luliconazole and excipients are revealed that there was no interaction between polymer and the drug, hence, they were compatible. Diethylene glycol monoethyl ether Transcutol P is selected as solvents to dissolve the Econazole because Luliconazole has higher solubility in Diethylene glycol monoethyl ether (Transcutol P) than the dimethyl sulfoxide and ethanol.

Based on saturation solubility study Isopropyl myristate, Liquid paraffin, Tween 20 and Tween 80 were selected for the formulation of emulsion.

The emulsion was formulated using HLB method for combination of two emulsifiers and characterised for its stability. Polymer Xanthan gum is used for the formulation of Emulgel and transparent gel. The Formulations of Emulgel and transparent gel meets the specification for evaluation parameters such as physical properties, spreadability, rheological properties, bioadhesive strength measurement, drug content determination, In-vitro release study, drug release kinetics.

Thus, it can be concluded that Econazole was proven to be a suitable candidate for formulating emulgel for topical delivery to achieve better patient compliance

REFERENCES

1. Kumar D, Singh J, Antil M and Kumar V: Emulgel-novel topical drug delivery system–a comprehensive review. Int J Pharm Sci Res 2016; 7(12): 4733-42.
2. Sunil Kumar Yadav, Manoj Kumar Mishra, Anupamaa Tiwari, Ashutosh Shukla. Emulgel: a new approach for enhanced topical drug delivery. Int J Curr Pharm Res 2017;9(1):15-19.
3. Shiva prasada N, Mutta SK, A Review on Emulgel, Asian Journal of Pharmaceutical Research and Development. 2021;9(4):147-150.
4. Singh D, Mital N, Kaur K. Topical Drug Delivery Systems: A Patent Review, Expert Opinion on Therapeutic Patents. Taylor & Francis Publisher, 2016; 26(02):213-228.
5. Bora A, Deshmukh S, Kapileswar S. Recent Advances in Semisolid Dosage Form. International Journal of Pharmaceutical Sciences and Research, 2014; 05(09):3594-3608.
6. Valle M, Zamorani M. Skin and Subcutaneous Tissue. In: Ultrasound of the Musculoskeletal System. Medical Radiology (Diagnostic Imaging). Springer, 2007; 01(01):19-43.
7. Hardenia A, Jayronia S, Jain S. Emulgel: An emergent tool in topical drug delivery. International journal of pharmaceutical sciences and research. 2014;5(5):1653-60.
8. Shraddha V. Mohite*, Anuradha K. Salunkhe, Suresh G. Sudke: Emulgel: A Novel Approach For Hydrophobic Drugs Am. J.PharmTech Res. 2019;9(02):208-224.
9. Kuller R, Saini S, Seth N, Rana AC, Emulgel: A surrogate approach for topical used hydrophobic drugs. Int J Pharm Bio Sci, 2011; 1(3): 117-128.
10. Aher SD, Banerjee SK, Gadhave MV, Gaikawad DD. Emulgel: A New Dosage Form For Topical Drug Delivery. International Journal of Institutional Pharmacy and Life Sciences, 2013; 3(3): 1-10.
11. Prathibha vegunta, T. Geetha Priya, Emulgel – A topical drug delivery system, Word journal of pharmaceutical research vol. 5, issue 7, 430-451.
12. Vikas S., Seema S., BaibhavJ. And Rana A. Emulgel: a new platform for topical drug delivery. International Journal of Pharma and Bio Sciences. 2012;( 1):3.
13. Shah A, Kamdar K, Shah R, Keraliya R, Emulgel: A Topical Preparation for Hydrophobic Drugs. Pharma Tech Medica, 2013;02(05):370-376.
14. Shah S, Badola B, Nayak B. Emulgel: Magnifying the application of topical drug delivery. Indian Journal of Pharmaceutical and Biological Research, 2017; 05(01):25-33.
15. Khambete H, Deveda P, Jain A, Vyas N, Jain S. Gellified Emulsion for Sustain Delivery of Itraconazole For Topical Fungal Diseases. International Journal of Pharmacy and Pharmaceutical Sciences, 2010; 02(01):104-112.
16. Sawant A, Mohite S. Formulation and Evaluation of Itraconazole Emulgel for Topical Drug Delivery. Asian Journal of Pharmacy and Technology, 2015; 05(02):91-96. 171.
17. Kansagra H Mallick S. Microemulsion-based antifungal gel of luliconazole for dermatophyte infections: formulation, characterization and efficacy studies. Journal of Pharmaceutical Investigation, 2016; 46(01):21–28.
18. Kumar D, Singh J, Antil M, Kumar V.Emulgel-novel topical drug delivery system – a comprehensive review. Int J Pharm Sci Res. 2016; 7(12):4733–4742.
19. Yadav SK, Mishra MK, Tiwari A, Shukla A. Emulgel: A new approach for enhanced topical drug delivery. Int J Curr Pharm Res. 2017; 9(1):15–19.
20. Mohite SV, Salunkhe AK, Sudke SG. Emulgel: A novel approach for hydrophobic drugs. Am J PharmTech Res. 2019; 9(2):208–224.
21. Kuller R, Saini S, Seth N, Rana AC. Emulgel: A surrogate approach for topical used hydrophobic drugs. Int J Pharm Bio Sci. 2011; 1(3):117–128.
22. Ahmad D, Upadhyay P, Ahsan W. Formulation and evaluation of sustained release antifungal emulgel of voriconazole. Int J Pharm Pharm Sci. 2017; 9(2):186–191.
23. Sawant A, Mohite S. Formulation and evaluation of Itraconazole Emulgel for topical drug delivery. Asian J Pharm Technol. 2015; 5(2):91–96.
24. Microemulsion-based antifungal gel of Luliconazole for dermatophyte infections: formulation, characterization and efficacy studies. Pharm Investig. 2016; 46(1):21–28.
25. Hardenia A, Jayronia S, Jain S.Emulgel: An emergent tool in topical drug delivery.  Int J Pharm Sci Res. 2014; 5(5):1653–1660.
26. Shah A, Kamdar K, Shah R, Keraliya R. Emulgel: A Topical Preparation for Hydrophobic Drugs. Pharma Tech Medica, 2013; 2(5): 370–376.
27. Shah S, Badola B, Nayak B. Emulgel: Magnifying the application of topical drug delivery. Indian J Pharm Biol Res, 2017; 5(1):25-33.