Exploring The Phytochemical Diversity of Hygrophila Polysperma Leaf Extracts with Varying Polarity Solvents

Abstract

The study investigates the phytochemical composition of Hygrophila polysperma leaf extract using polarity-based solvents, aiming to identify bioactive compounds with potential medicinal properties. The leaves of Hygrophila polysperma were extracted using solvents of varying polarities) low to high) such as Petroleum ether (low polarity), Chloroform, Ethyl acetate, Ethanol and water (high polarity). Phytochemical screening exposed the existence of several bioactive composites, such as alkaloids, flavonoids, saponins, phenols, tannins, glycosides, and terpenoids, with varying concentrations depending on the solvent used. The ethanol extract exhibited the highest diversity of phytochemicals, particularly phenolic compounds, tannins, alkaloids, carbohydrates, terpenoids and flavonoids, known for their antioxidant property. The chloroform extract showed a moderate presence of alkaloids and some quantity of glycosides. The results confirm that Hygrophila polysperma contains a range of bioactive compounds to treat various ailments, including in the treatment of infertility. Further studies, including isolation and characterization of individual bioactive compounds, are recommended to explore their pharmacological potential. As part of preliminary analysis of raw extracts or fractions from various sources, online isolation and discovery of natural substances, and chemical taxonomic studies.

Keywords

Introduction

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Extraction of plant material:

DISCUSSION:

The study suggests that Hygrophila polysperma leaf contains a range of bioactive compounds, which could be beneficial for human health. These compounds might have potential therapeutic properties, particularly in the treatment of infertility. The presence of these bioactive substances supports the traditional use of the whole plant in treating various ailments. The findings reinforce the idea that natural plant products often contain compounds that modern science is just beginning to understand and validate. The next phase of the study will likely focus on isolating and purifying the specific bioactive compounds responsible for the observed medicinal effects. This could involve using techniques like chromatography (e.g., HPLC or TLC) to separate the different compounds and identify their structures.

CONCLUSION:

The phytochemical analysis of Hygrophila polysperma has revealed the presence of a variety of bioactive compounds that are likely responsible for the plant's therapeutic properties. These compounds include alkaloids, flavonoids, phenolic acids, glycosides, and other secondary metabolites, which have demonstrated potential medicinal value in various health conditions. As a next step, isolating and purifying these bioactive compounds will be essential to fully understand their molecular structure, potency, and mechanisms of action. Further in vitro and in vivo studies will be necessary to evaluate their effectiveness and safety in human health applications. This research not only validates the traditional medicinal practices surrounding Hygrophila polysperma, but it also opens avenues for future pharmaceutical and therapeutic applications.

ACKNOWLEDGEMENT:

I would like to express my heartfelt gratitude to our beloved Dean, guide, co-guide, advisory committee members and our lab technicians and for contributed to the successful completion of this study on the phytochemical analysis of Hygrophila polysperma.

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